Quantitative analysis of the dentin adhesive interface by Auger spectroscopy
Quantitative analysis of the dentin adhesive interface by Auger spectroscopy
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DOI：
10.1177/00220345960750040501
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发表时间：
1996-04-01
影响因子：
7.6
通讯作者：
Chappelow, CC
中科院分区：
文献类型：
--
作者：
Eick, JD;Miller, RG;Chappelow, CC
牙本质粘合剂粘合的最终成功在很大程度上取决于牙齿和粘合剂之间界面的具体条件。大多数当前的牙本质粘合剂系统使用某种预处理来使牙本质表面的前几微米脱矿质，留下胶原蛋白网状结构，粘合树脂可以渗透、渗透和聚合。本实验测试的一般假设是，粘合剂树脂向脱矿质区域的渗透和分布是用作粘合剂应用预处理的调节剂的函数。对四种市售粘合剂系统进行了改进，加入硫代甲基丙烯酸羟乙酯 (HETMA)，这是一种硫取代的、可追踪的甲基丙烯酸 2-羟乙酯 (HEMA) 类似物，从而可以通过能量色散 X 射线光谱 (EDS) 定性测量处理过的牙本质基质中单体的数量和分布，并通过俄歇电子能谱 (AES) 定量测量。研究的牙本质预处理是：（1）10％柠檬酸/3％氯化铁，（2）10％马来酸，（3）2.5％硝酸，和（4）HEMA与磷酸酯的醇溶液。这些预处理应用于刚拔出的牙齿，这些牙齿已被切片以暴露牙本质并研磨以模拟涂抹层。进行适当的预处理后，将 10% (v/v) HETMA 丙酮溶液涂覆到表面，然后涂覆相应的粘合树脂，然后进行聚合。然后对样品进行处理，以便通过扫描透射电子显微镜 (STEM)、AES 和 STEM/EDS 分析进行观察。结果表明，通过本文研究的四种预处理，HETMA 渗透牙本质表面的能力存在显着差异。这项研究还表明，AES 和 STEM/EDS 可以以相关方式用于确定处理过的牙本质表面内或附近的 HETMA 分布。
The ultimate success of a dentin adhesive bond is dependent in large part on specific conditions at the interface between the tooth and the adhesive. Most current dentin adhesive systems use some sort of pre-treatment to demineralize the first few microns of the dentin surface, leaving a meshwork of collagen into which the adhesive resin can penetrate, infiltrate, and polymerize. The general hypothesis tested in this experiment was that the penetration and distribution of adhesive resin into the demineralized zone are a function of the conditioner used as a pre-treatment for the adhesive application. Four commercially available adhesive systems were modified to incorporate hydroxyethylthiomethacrylate (HETMA), a sulfur-substituted, traceable analogue of 2-hydroxyethylmethacrylate (HEMA), thereby allowing for a qualitative measurement of the amount and distribution of monomer in the treated dentin substrate by energy-dispersive x-ray spectroscopy (EDS) and a quantitative measurement by Auger electron spectroscopy (AES). The dentin pre-treatments investigated were: (1) 10% citric acid/3% ferric chloride, (2) 10% maleic acid, (3) 2.5% nitric acid, and (4) an alcoholic solution of HEMA with a phosphorus acid ester. These pre-treatments were applied to freshly extracted teeth that had been sectioned to expose the dentin and ground to simulate the smeared layer. After the appropriate pre-treatment was applied, a 10% (v/v) solution of HETMA in acetone was applied to the surface, followed by the corresponding adhesive resin, which was then polymerized. The samples were then processed for observation by scanning transmission electron microscopy (STEM), AES, and STEM/EDS analysis. The results indicated significant differences in the ability of HETMA to penetrate the dentin surface conditioned by the four pretreatments investigated here. This study also demonstrated that AES and STEM/EDS could be used in a correlative fashion to determine the distribution of HETMA within or adjacent to the treated dentin surface.