Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways
Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways
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DOI：
10.1039/c5gc01347j
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发表时间：
2015-01-01
期刊：
影响因子：
9.8
通讯作者：
Bugg, Timothy D. H.
中科院分区：
文献类型：
--
作者：
Mycroft, Zoe;Gomis, Maria;Bugg, Timothy D. H.
如果能够开发出有效的解聚方法，杂聚物木质素代表了用于生产可再生芳香族化学品的未开发资源。在这项工作中，红球菌 jostii RHA1 中降解芳香族木质素分解产物的代谢途径被重新设计，以产生可用于生物塑料合成的芳香族二羧酸产物。原儿茶酸通常通过邻位裂解代谢为 β-酮己二酸途径。将原儿茶酸 4,5-双加氧酶或原儿茶酸 2,3-双加氧酶的重组基因插入 R. jostii RHA1，然后对额外二醇裂解产物进行氨环化，在含有 1% 小麦秸秆的基本培养基上生长时，产生吡啶 2,4-二甲酸或吡啶 2,5-二甲酸生物产物，产量为 80-125 mg L-1木质纤维素。
The heteropolymer lignin represents an untapped resource for production of renewable aromatic chemicals, if efficient depolymerisation methods can be developed. In this work, the metabolic pathways in Rhodococcus jostii RHA1 for degradation of aromatic lignin breakdown products are re-routed, in order to generate an aromatic dicarboxylic acid product that could be used for bioplastic synthesis. Protocatechuic acid is normally metabolised via ortho-cleavage to the beta-keto-adipate pathway. Insertion of recombinant genes for protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase into R. jostii RHA1, followed by ammonia cyclisation of the extradiol cleavage products, generates pyridine 2,4-dicarboxylic acid or pyridine 2,5-dicarboxylic acid bioproducts in yields of 80-125 mg L-1 when grown on minimal media containing 1% wheat straw lignocellulose.