Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs
Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs
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DOI：
10.1111/j.1349-7006.2010.01723.x
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发表时间：
2010-12-01
期刊：
影响因子：
5.7
通讯作者：
Wu, Benjamin M.
中科院分区：
文献类型：
--
作者：
Ho, Won Jin;Pham, Edward A.;Wu, Benjamin M.
癌症疗法的发展需要在动物试验和随后的临床试验之前对药物的体外疗效进行彻底的评估。因此，三维（3-D）体外模型已被研究用于药物筛选。在这项研究中，我们开发了一种新的体外模型，其中多细胞聚集体或球体被纳入三维多孔支架。耐药试验表明，球形支架比单层培养、平面基质球形支架和分散细胞支架具有更高的耐药能力。此外，球形种子支架显示出更高的乳酸生成导致酸中毒，以及更高的血管生成因子表达。这些数据表明，球形种子三维支架可能作为一个有用的体外系统筛选癌症治疗。[j] .癌症科学，2010;31(2):357 - 357。
Development of cancer therapeutics requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Three-dimensional (3-D) in vitro models have therefore been investigated for drug screening. In this study, we have developed a novel in vitro model in which multicellular aggregates, or spheroids, were incorporated into 3-D porous scaffolds. Drug resistance assays showed that spheroid-seeded scaffolds have much higher drug resistance than monolayer cultures, spheroids on flat substrates, or scaffolds seeded with dispersed cells. Furthermore, spheroid-seeded scaffolds demonstrated higher lactate production leading to acidosis, and higher expression of angiogenic factors. These data suggest that the spheroid-seeded 3-D scaffolds might serve as a useful in vitro system for screening cancer therapeutics. (Cancer Sci 2010; 101: 2637-2643).