<i>ccr7</i> affects both morphogenesis and differentiation during early <i>Xenopus</i> embryogenesis
<i>ccr7</i> affects both morphogenesis and differentiation during early <i>Xenopus</i> embryogenesis
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<i>ccr7</i> 影响非洲爪蟾早期胚胎发生过程中的形态发生和分化
DOI：
10.1111/dgd.12790
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Shibuya Hiroshi
中科院分区：
文献类型：
--
作者：
Goto Toshiyasu;Michiue Tatsuo;Shibuya Hiroshi
在免疫系统建立之前，趋化因子在早期胚胎发生中发挥重要作用，包括形态发生和细胞分化。我们对非洲爪蟾 CC 型趋化因子受体 7 S (ccr7.S) 进行了表征，以阐明其在早期发育过程中的作用。ccr7 转录本在早期胚胎中普遍存在。 ccr7.Sin 抑制原肠胚形成和 ccr7.SmRNA 注射胚胎的背侧过度表达具有短轴和广泛开放的神经褶皱。由于注射胚胎的凯勒三明治外植体伸长良好，ccr7.S可能会影响细胞迁移，但不会影响会聚延伸运动。 Ventralccr7.Sover表达诱导原肠胚期胚胎中的次轴和chrd.1上调。动物帽测定显示神经和水泥腺标记基因在后期的表达增加。 Ccr7.S 敲低减少了chrd.1 的表达并抑制了背侧的原肠胚形成。我们的研究结果表明ccr7.S在形态发生运动和细胞分化中发挥重要作用。
Chemokines play important roles in early embryogenesis, including morphogenesis and cell differentiation, before the immune system is established. We characterizedXenopus laevisCC‐type chemokine receptor 7 S (ccr7.S) to clarify its role during early development.ccr7transcripts were detected ubiquitously in early embryos. Dorsal overexpression ofccr7.Sinhibited gastrulation, andccr7.SmRNA‐injected embryos had short axes and widely opened neural folds. Because the Keller sandwich explants of the injected embryos elongated well,ccr7.Smight affect cell migration, but not convergent extension movements. Ventralccr7.Soverexpression induced secondary axes andchrd.1upregulation in gastrula‐stage embryos. Animal cap assays showed increased expression of neural and cement gland marker genes at later stages. Ccr7.S knockdown reducedchrd.1expression and inhibited gastrulation at the dorsal side. Our findings suggest thatccr7.Splays important roles in morphogenetic movement and cell differentiation.