Molecular marker assisted gene stacking for biotic and abiotic stress resistance genes in an elite rice cultivar.
Molecular marker assisted gene stacking for biotic and abiotic stress resistance genes in an elite rice cultivar.
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DOI：
10.3389/fpls.2015.00698
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发表时间：
2015
影响因子：
5.6
通讯作者：
Rao GJ
中科院分区：
文献类型：
--
作者：
Das G;Rao GJ
由于各种生物胁迫，如白叶枯病（BB）、瘿蚊（昆虫）和稻瘟病（疾病）以及淹没和盐度等非生物胁迫造成的严重产量损失，严重制约了全世界水稻的生产力。最有效和可靠的管理压力的方法是通过经济和环境友好的方法增强宿主的抵抗力。本研究利用标记辅助选择（MAS）技术，成功地将已释放的改良Lalat品种CRMAS2621-7-1的基因/ qtl进行了基因/ qtl的转形，使其具有抗稻瘟病（Pi2, Pi9）、胆蚊（Gm1, Gm4）、淹水（Sub1）和耐盐（Saltol）的能力。该改良Lalat品种已添加了3个抗BB基因xa5、xa13和Xa21，以补充改良Lalat中存在的Xa4基因。分子分析显示，所有标记目标性状的标记在供体和受体亲本之间存在明显的多态性。在BC3F1代之前，采用常规回交育种方法，从BC1F1代开始，每一步采用标记辅助选择，用分子标记监测目标等位基因的转移。将具有目标基因/ qtl的不同BC3F1s进行内交，将所有10个抗逆性/耐受性基因/ qtl杂交到单个植株/品系中。获得了不同组合的抗性/抗性基因纯合子植株。对BC3F3品系进行了农艺性状和品质性状的鉴定，选育了有发展前景的后代品系。基于SSR进行背景选择。大部分基因金字塔线在形态、籽粒品质性状和基于SSR的背景选择上都与回亲本高度相似。在所有测试的基因金字塔中，有2个品系具有所有10个抗性/耐受性基因，并且对5个目标逆境表现出足够的抗性/耐受性。该研究表明，MAS具有将多个基因堆叠成单系的潜力，亲本基因组恢复程度高。
Severe yield loss due to various biotic stresses like bacterial blight (BB), gall midge (insect) and Blast (disease) and abiotic stresses like submergence and salinity are a serious constraint to the rice productivity throughout the world. The most effective and reliable method of management of the stresses is the enhancement of host resistance, through an economical and environmentally friendly approach. Through the application of marker assisted selection (MAS) technique, the present study reports a successful pyramidization of genes/QTLs to confer resistance/tolerance to blast (Pi2, Pi9), gall Midge (Gm1, Gm4), submergence (Sub1), and salinity (Saltol) in a released rice variety CRMAS2621-7-1 as Improved Lalat which had already incorporated with three BB resistance genes xa5, xa13, and Xa21 to supplement the Xa4 gene present in Improved Lalat. The molecular analysis revealed clear polymorphism between the donor and recipient parents for all the markers that are tagged to the target traits. The conventional backcross breeding approach was followed till BC3F1 generation and starting from BC1F1 onwards, marker assisted selection was employed at each step to monitor the transfer of the target alleles with molecular markers. The different BC3F1s having the target genes/QTLs were inter crossed to generate hybrids with all 10 stress resistance/tolerance genes/QTLs into a single plant/line. Homozygous plants for resistance/tolerance genes in different combinations were recovered. The BC3F3 lines were characterized for their agronomic and quality traits and promising progeny lines were selected. The SSR based background selection was done. Most of the gene pyramid lines showed a high degree of similarity to the recurrent parent for both morphological, grain quality traits and in SSR based background selection. Out of all the gene pyramids tested, two lines had all the 10 resistance/tolerance genes and showed adequate levels of resistance/tolerance against the five target stresses. The study demonstrates the potential of MAS for stacking of several genes into a single line with a high degree of parental genome recovery.