Langerhans cells exhibit low responsiveness to double-stranded RNA
Langerhans cells exhibit low responsiveness to double-stranded RNA
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DOI：
10.1016/j.bbrc.2004.05.059
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Tamaki, K
中科院分区：
文献类型：
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作者：
Fujita, H;Asahina, A;Tamaki, K
双链RNA（dsRNA）是由Toll样受体3（TLR 3）识别的病毒产物，是树突状细胞（DC）的有效激活剂。我们比较了朗格汉斯细胞（LC）和脾脏CD 11 c（+）DC，并研究了对dsRNA的反应性。我们使用淘洗方法制备了高度纯化的LC（>95%）。TLR 3 mRNA在LC、脾DC和角质形成细胞（KC）中表达。经Poly（I：C）刺激后，LC和脾DC中IFN-β mRNA的表达增强。然而，LC对Poly（I：C）的细胞因子/趋化因子产生远低于脾DC。此外，Poly（I：C）诱导脾DC进一步成熟，但在LC中不诱导。最后，我们发现小鼠KC细胞系PAM 212在Poly（I：C）刺激下产生大量IL-1 α，并且IL-1 α促进LC的成熟。这些数据共同表明LC对dsRNA表现出低响应性。KC可能主要通过细胞因子（如IL-1 α）的产生引发皮肤中的抗病毒免疫反应。(C)2004年爱思唯尔公司All rights reserved.
Double-stranded RNA (dsRNA) is a viral product recognized by Toll-like receptor 3 (TLR3), and it is a potent activator of dendritic cells (DC). We compared Langerhans cells (LC) and splenic CD11c(+) DC and investigated the responsiveness to dsRNA. We prepared highly purified LC (>95%) using the panning method. TLR3 mRNA was expressed in LC, splenic DC, and keratinocytes (KC). The expression of IFN-beta mRNA was enhanced in LC and splenic DC by Poly(I:C) stimulation. However, cytokine/chemokine production in response to Poly(I:C) by LC was much lower than that by splenic DC. In addition, Poly(I:C) induced further maturation in splenic DC, but not in LC. Finally, we found that the mouse KC cell line, PAM212, produced a great amount of IL-1alpha by Poly(I:C) stimulation, and that IL-1alpha promoted the maturation of LC. These data altogether indicate that LC exhibit low responsiveness to dsRNA. It is possible that KC may primarily trigger anti-viral immune responses in the skin via cytokine production such as IL-1alpha. (C) 2004 Elsevier Inc. All rights reserved.