Application of mRNA Arrays for the Production of mCherry Reporter-Protein Arrays for Quantitative Gene Expression Analysis.
Application of mRNA Arrays for the Production of mCherry Reporter-Protein Arrays for Quantitative Gene Expression Analysis.
复制标题
DOI：
10.1021/acssynbio.8b00266
复制
发表时间：
2019-01
影响因子：
4.7
通讯作者：
Masoud Norouzi;A. Pickford;Louise E. Butt;H. Vincent;A. J. Callaghan
中科院分区：
文献类型：
--
作者：
Masoud Norouzi;A. Pickford;Louise E. Butt;H. Vincent;A. J. Callaghan
开发可精确和可预测地控制基因表达的可编程调节器是合成生物学的主要目标。因此，能够定量分析基因表达的所有组分的快速高通量生物化学方法将在调节器性能的表征和优化中具有价值。在这项研究中，我们展示了一种新的应用程序的RNA阵列，涉及生产的蛋白质阵列，基因表达分析。该方法能够同时定量基因表达的转录和转录后/翻译组分，并且它还允许评估多个调节器的正交性。我们使用我们的方法直接比较一系列先前表征的合成转录后核糖核酸调节子的性能，从而证明其在合成调控模块的开发和基因表达调控的评价中的实用性。
The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene expression would be of value in the characterization and optimization of regulator performance. In this study we demonstrate a novel application of RNA arrays, involving the production of reporter-protein arrays, to gene expression analysis. This method enables simultaneous quantification of both the transcription and post-transcription/translation components of gene expression, and it also allows the assessment of the orthogonality of multiple regulators. We use our method to directly compare the performance of a series of previously characterized synthetic post-transcriptional riboregulators, thus demonstrating its utility in the development of synthetic regulatory modules and evaluation of gene expression regulation in general.