PRIMARY STRUCTURE AND EXPRESSION OF A FUNCTIONAL HUMAN GLUCOCORTICOID RECEPTOR CDNA
PRIMARY STRUCTURE AND EXPRESSION OF A FUNCTIONAL HUMAN GLUCOCORTICOID RECEPTOR CDNA
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DOI：
10.1038/318635a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
EVANS, RM
中科院分区：
文献类型：
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作者：
HOLLENBERG, SM;WEINBERGER, C;EVANS, RM
编码人糖皮质激素受体的互补DNA的鉴定预测了两种蛋白质形式，777（α）和742（β）氨基酸，其羧基末端不同。这些蛋白质含有每个富含半胱氨酸的区域的半胱氨酸/赖氨酸，其可以限定DNA结合结构域。体外合成的纯放射性标记糖皮质激素受体具有免疫反应性，并具有天然糖皮质激素受体的固有类固醇结合活性特征。
Identification of complementary DNAs encoding the human glucocorticoid receptor predicts two protein forms, of 777 (.alpha.) and 742 (.beta.) amino acids, which differ at their carboxy termini. The proteins contain a cysteine/lysine per arginine-rich region which may define the DNA-binding domain. Pure radiolabelled glucocorticoid receptor, synthesized in vitro, is immunoreactive and possess intrinsic steroid-binding activity characteristic of the native glucocorticoid receptor.