Overexpression of the neuropilin 1 (NRP1) gene correlated with poor prognosis in human glioma.
Overexpression of the neuropilin 1 (NRP1) gene correlated with poor prognosis in human glioma.
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发表时间：
2004-03
影响因子：
2
通讯作者：
H. Osada;T. Tokunaga;M. Nishi;H. Hatanaka;Y. Abe;A. Tsugu;H. Kijima;H. Yamazaki;Y. Ueyama;Masato Nakamura
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文献类型：
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作者：
H. Osada;T. Tokunaga;M. Nishi;H. Hatanaka;Y. Abe;A. Tsugu;H. Kijima;H. Yamazaki;Y. Ueyama;Masato Nakamura
我们研究了神经纤毛蛋白（NRP）基因的表达是否与胶质瘤的临床病理特征相关。采用真实的时间反转录聚合酶链反应（真实的timeRT-PCR）和免疫组化方法检测37例脑胶质瘤中血管内皮生长因子（VEGF）-A、Flt-1、KDR、NRP 1和NRP 2基因的表达。用抗CD 34抗体检测肿瘤血管计数。与正常脑组织样品相比，肿瘤组织中NRP 1 mRNA的过表达显著更高。高级别胶质瘤中NRP 1基因表达显著增高（p=0.0015）。NRP 1过表达的胶质瘤患者的预后较差（p=0.0202）比那些没有这样的过表达。免疫组化法检测NRP 1在胶质瘤细胞中的表达。VEGF-A和VEGFR过表达与临床病理特征（包括NRP表达）无关。这些结果表明，NRP 1过表达，而不是VEGF-A或VEGFR，有助于肿瘤的进展，并具有临床意义的胶质瘤。
We studied whether the expression of the Neuropilin (NRP) gene was correlated with clinicopathological features in glioma. We examined the gene expression of vascular endothelial growth factor (VEGF)-A, Flt-1, KDR, NRP1 and NRP2 in 37 gliomas by real time reverse transcriptase PCR (real time RT-PCR) as well as immunohistochemical analysis. The vascular counts of each tumor were evaluated by anti-CD34 antibody. NRP1 mRNA overexpression was significantly higher in neoplastic tissue compared to normal brain tissue samples. The higher grade of glioma overexpressed the NRP1 gene significantly (p=0.0015). The glioma patients with NRP1 overexpression showed a poorer prognosis (p=0.0202) than those without such overexpression. NRP1 was observed in the glioma cells by immunohistochemical analyses. VEGF-A and VEGFR overexpression did not show any correlation with the clinicopathological features, including NRP expression. These results suggest that NRP1 overexpression, rather than VEGF-A or VEGFR, contributes to tumor progression and has clinical significance for glioma.