A transcriptomic (RNA-seq) analysis of genes responsive to both cadmium and arsenic stress in rice root
A transcriptomic (RNA-seq) analysis of genes responsive to both cadmium and arsenic stress in rice root
复制标题
水稻根部镉和砷胁迫响应基因的转录组 (RNA-seq) 分析
DOI：
10.1016/j.scitotenv.2019.02.281
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发表时间：
2019-05-20
影响因子：
9.8
通讯作者：
Yi, Jicai
中科院分区：
文献类型：
--
作者：
Huang, Yingmei;Chen, Huiqiong;Yi, Jicai
镉(Cd)和砷(As)是水稻中的非必需有毒元素，常同时存在于受污染的稻田土壤中。为了了解水稻对Cd和As毒害是否存在共同的分子响应机制，在水培条件下对30天的水稻幼苗分别暴露Cd和As3+7d。根转录分析表明，水稻根系中共有2224个基因对Cd胁迫有反应，1503个基因对As胁迫有反应。在这些基因中，有841个基因对两种压力源都有反应。Cd和As胁迫的共同基因与氧化还原控制、胁迫响应、转录调控、跨膜运输、信号转导以及大分子和硫化物的生物合成和代谢有关。在Cd和As单独或联合暴露3d和7d的植物中，qRT-PCR验证表明谷胱甘肽代谢相关基因Os09G0367700比对照显著上调，并在Cd和As联合胁迫下具有正协同效应。此外，氧化还原调控相关基因Os06g0216000、Os07g0638300和Os01g0294500、谷胱甘肽代谢相关基因Os01g0530900、细胞壁生物发生相关基因Os05g0247800、Os11g0592000和Os03g0416200、表达调控相关基因Os07g0597200和Os02g0168200以及跨膜运输相关基因Os04g0524500与对照相比也有显著差异，表现出协同效应。我们在水稻中发现了一组同时响应Cd和As3+胁迫的新基因，这可能对减轻共污染土壤的毒性有价值。这些结果也加深了对多金属/固体胁迫反应的分子机制的理解，并可能用于水稻品种的遗传改良。(C)2019爱思唯尔B.V.保留所有权利。
Cadmium (Cd) and arsenic (As) are nonessential and toxic elements in rice that often occur together in contaminated paddy field soils. To understand whether rice has a common molecular response mechanism against Cd and As toxicity, 30-day seedlings (Oryza sativa L. indica) were exposed separately to Cd and As3+ in hydroponic cultures for up to 7 days. Root transcriptomic analysis of plants exposed to Cd and As for 3 days revealed that a total of 2224 genes in rice roots responded to Cd stress, while 1503 genes responded to As stress. Of these, 841 genes responded to both stressors. The genes in common to Cd and As stress were associated with redox control, stress response, transcriptional regulation, transmembrane transport, signal transduction, as well as biosynthesis and metabolism of macromolecules and sulfur compounds. In plants exposed to Cd and As separately or in combination for 3 and 7 days, qRT-PCR verification revealed that the glutathione metabolism associated gene Os09g0367700 was significantly up-regulated with respect to unexposed controls and had a positive synergistic effect under combined Cd and As stress. In addition, the redox control related genes Os06g0216000, Os07g0638300 and Os01g0294500, the glutathione metabolism related gene Os01g0530900, the cell wall biogenesis related genes Os05g0247800, Os11g0592000 and Os03g0416200, the expression regulation related genes Os07g0597200 and Os02g0168200, and the transmembrane transport related genes Os04g0524500, also varied significantly with respect to an unexposed control and displayed synergistic effects after 7 days of simultaneous exposure to Cd and As. Our identification of a novel set of genes in rice which responded to both Cd and As3+ stress may be of value in mitigating the toxicity of co-contaminated soils. These results also provide a deeper understanding of the molecular mechanisms involved in response to multi-metal/loids stress, and may be used in the genetic improvement of rice varieties. (C) 2019 Elsevier B.V. All rights reserved.