Effect of UV-B Irradiation on Bioactive Compounds of Red Perilla (Perilla frutescens (L.) Britton) Cultivated in a Plant Factory with Artificial Light
Effect of UV-B Irradiation on Bioactive Compounds of Red Perilla (Perilla frutescens (L.) Britton) Cultivated in a Plant Factory with Artificial Light
复制标题
DOI：
10.3390/horticulturae8080725
复制
发表时间：
2022-08
期刊：
影响因子：
3.1
通讯作者：
H. Yoshida;Kanae Shimada;S. Hikosaka;E. Goto
中科院分区：
文献类型：
--
作者：
H. Yoshida;Kanae Shimada;S. Hikosaka;E. Goto
在本研究中，我们研究了收获前 3 天的 UV-B 照射对红紫苏 (Perilla frutescens L.) 中迷迭香酸 (RA) 和花青素积累的影响，以及与苯丙素和类黄酮生物合成途径相关的基因表达的影响。实验1中，播种后60天（DAS）的幼苗在16 h光照周期下接受0（对照）、6和10 W m−2的UV-B照射；实验2中，45 DAS的幼苗接受UV-B照射0（对照），连续照射4 W m−2，16 h照射6 W m−2。 10 W m−2 的紫外线照射 16 小时会对叶色产生负面影响，而 6 W m−2 的照射则增强 RA 生物合成和抗氧化能力。与对照相比，4 W m−2 的连续 UV-B 照射使 RA 浓度增加了 92%；然而，这种效果比6 W m−2 UV-B照射16小时的效果要小，比对照高141%，并且在黑暗时期对UV介导的ROS过量产生的抗氧化能力较低。结果表明，6 W·m−2的UV-B照射16 h适合提高红紫苏的RA浓度和含量。
In this study, we investigated the effect of UV-B irradiation 3 days prior to harvest, on the accumulation of rosmarinic acid (RA) and anthocyanin, and the expression of genes related to phenylpropanoid and flavonoid biosynthetic pathways, in red perilla (Perilla frutescens L.). In experiment 1, seedlings at 60 days after sowing (DAS) were subjected to UV-B irradiation at 0 (control), 6, and 10 W m−2 under a 16 h light period; while in experiment 2, seedlings at 45 DAS were subjected to UV-B irradiation at 0 (control), 4 W m−2 at continuous irradiation, and 6 W m−2 at 16 h irradiation. UV irradiation of 10 W m−2 for 16 h negatively affected leaf color, while irradiation at 6 W m−2 enhanced RA biosynthesis and antioxidant capacity. Continuous UV-B irradiation of 4 W m−2 increased the RA concentration by 92% compared to the control; however, this effect was smaller than that of UV-B irradiation at 6 W m−2 for 16 h, 141% higher than that of the control and had a lower antioxidant capacity against UV-mediated ROS overproduction during the dark period. Results demonstrate that 6 W m−2 of UV-B irradiation for 16 h is suitable for enhancing the RA concentration and content of red perilla.