Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug. resistant pathogens
Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug. resistant pathogens
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DOI：
10.1016/j.procbio.2013.05.011
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发表时间：
2013-07-01
影响因子：
4.4
通讯作者：
Lim, Seung Taik
中科院分区：
文献类型：
--
作者：
Kanmani, Paulraj;Lim, Seung Taik
采用一种绿色、简单、有效的方法，利用细菌胞外多糖作为还原剂和稳定剂合成有效的银纳米颗粒（SNPs）。采用紫外可见光谱、透射电子显微镜、能量色散X射线分析、X射线衍射和傅立叶变换红外光谱分析对合成的SNPs进行表征。SNPs的形状不同，是多分散的，平均直径为10 nm，范围为2至15 nm，在室温下稳定长达2个月。使用琼脂孔扩散法分析SNP对细菌和真菌病原体的抗微生物活性。对所有细菌病原体均观察到剂量依赖性抑制。铜绿假单胞菌和克雷伯菌是多重耐药菌。肺炎被发现比食源性病原体单核细胞增生李斯特菌更容易受到SNP的影响。真菌Aspergillus spp.与青霉属相比，显示出最大的抑制区。这些结果表明，胞外多糖稳定的SNP可用作各种生物医学应用的抗微生物剂。(C)2013爱思唯尔有限公司保留所有权利。
A green, simple, and effective approach was performed to synthesize potent silver nanoparticles (SNPs) using bacterial exopolysaccharide as both a reducing and stabilizing agent. The synthesized SNPs were characterized using UV-vis spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis, X-ray diffraction, and Fourier-transform-infrared spectra analyses. The SNPs varied in shape and were multidispersed with a mean diameter of 10 nm ranging from 2 to 15 nm and were stable up to 2 months at room temperature. The antimicrobial activity of the SNPs was analyzed against bacterial and fungal pathogens using the agar well diffusion method. Dose dependent inhibition was observed for all bacterial pathogens. The multidrug resistant pathogens P. aeruginosa and K. pneumonia were found to be more susceptible to the SNPs than the food borne pathogen L monocytogenes. The fungi Aspergillus spp. exhibited a maximum zone of inhibition compared to that of Penicillum spp. These results suggest that exopolysaccharide-stabilized SNPs can be used as an antimicrobial agent for various biomedical applications. (C) 2013 Elsevier Ltd. All rights reserved.