Quantitative kinetic modelling and mapping of cerebral glucose transport and metabolism using glucoCESL MRI.
Quantitative kinetic modelling and mapping of cerebral glucose transport and metabolism using glucoCESL MRI.
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DOI：
10.1177/0271678x221108841
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发表时间：
2022-11
影响因子：
6.3
通讯作者：
Matthews, Julian C.
中科院分区：
文献类型：
--
作者：
Dickie, Ben R.;Jin, Tao;Wang, Ping;Hinz, Rainer;Harris, William;Boutin, Herve;Parker, Geoff J. M.;Parkes, Laura M.;Matthews, Julian C.
化学交换自旋锁定磁共振成像可以在高空间分辨率(即低于0.2 mm~3体素)下定位大脑中葡萄糖的局部摄取和利用。我们提出了两个定量动力学模型来描述葡萄糖诱导的组织R1ρ的变化，并将它们应用于在荷瘤和健康大鼠中获得的葡萄糖CESL磁共振数据。在假定葡萄糖转运是饱和的情况下，正常组织测得的最大转运能力(Tmax)为3.2mmol0.6 /m in/m L，半饱和常数(Kt)为8.8m o l± 2.2m o l/m in mm，葡萄糖消耗代谢率(M Rglc)为0.21 ± 0.13 m o l/m in/m L，脑血容量(V B)为0.006 ±0.005 m L/m L。肿瘤Tmax = 7.1Min/m L，Kt = 14 ± 1.7m mm，MRglc = 0.22 ± 0.09 µm o l/m in/m L，vb = 0.030 ± 0.035 m L/m L。肿瘤组织的Tmax和Kt显著高于正常组织(p = 0.006和p = 0.011)。当假定葡萄糖摄取也是通过自由扩散时，正常组织和肿瘤组织的自由扩散速率(Kd)分别为0.061 ± 0.017 m L/m in/m L和0.12 ± 0.042 m L/m in/m L。这些参数估计值与使用其他方法(例如核磁共振光谱学)获得的文献值很好地吻合。
Chemical-exchange spin-lock (CESL) MRI can map regional uptake and utilisation of glucose in the brain at high spatial resolution (i.e sub 0.2 mm3 voxels). We propose two quantitative kinetic models to describe glucose-induced changes in tissue R1ρ and apply them to glucoCESL MRI data acquired in tumour-bearing and healthy rats. When assuming glucose transport is saturable, the maximal transport capacity (Tmax) measured in normal tissue was 3.2 ± 0.6 µmol/min/mL, the half saturation constant (Kt) was 8.8 ± 2.2 mM, the metabolic rate of glucose consumption (MRglc) was 0.21 ± 0.13 µmol/min/mL, and the cerebral blood volume (vb) was 0.006 ± 0.005 mL/mL. Values in tumour were: Tmax = 7.1 ± 2.7 µmol/min/mL, Kt = 14 ± 1.7 mM, MRglc = 0.22 ± 0.09 µmol/min/mL, vb = 0.030 ± 0.035 mL/mL. Tmax and Kt were significantly higher in tumour tissue than normal tissue (p = 0.006 and p = 0.011, respectively). When assuming glucose uptake also occurs via free diffusion, the free diffusion rate (kd) was 0.061 ± 0.017 mL/min/mL in normal tissue and 0.12 ± 0.042 mL/min/mL in tumour. These parameter estimates agree well with literature values obtained using other approaches (e.g. NMR spectroscopy).