Osmotic swelling activates intermediate-conductance Cl- channels in human intestinal epithelial cells.
Osmotic swelling activates intermediate-conductance Cl- channels in human intestinal epithelial cells.
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渗透膨胀激活人肠上皮细胞中的中间电导 Cl- 通道。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
M. Tominaga
中科院分区：
文献类型：
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作者：
Y. Okada;C. Petersen;M. Kubo;S. Morishima;M. Tominaga
在人肠上皮细胞系的渗透膨胀过程中，仅当同时观察到失活的全细胞 Cl- 电流时，在施加大的正电位时，才能在细胞附着的斑块中观察到 Cl- 通道的逐步关闭单一事件。随着正指令脉冲的增加，闭合过程变得更快。单一电导在 60 mV 时约为 32 pS，在 +100 mV 时约为 37 pS，在 +140 mV 时约为 47 pS，表现出向外整流。
During osmotic swelling of a human intestinal epithelial cell line, stepwise closing unitary events of Cl- channels could be observed in cell-attached patches upon application of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed. The closing process became faster with increasing positive command pulses. The unitary conductance was around 32 pS at 60 mV, 37 pS at +100 mV, and 47 pS at +140 mV exhibiting outward rectification.