Expression of cathepsin-K in gingival crevicular fluid of patients with periodontitis
Expression of cathepsin-K in gingival crevicular fluid of patients with periodontitis
复制标题
DOI：
10.1016/j.archoralbio.2007.01.006
复制
发表时间：
2007-09-01
影响因子：
3
通讯作者：
Otogoto, Junichi
中科院分区：
文献类型：
--
作者：
Mogi, Makio;Otogoto, Junichi
组织蛋白酶K是一种高表达的半胱氨酸蛋白酶，在骨重建和骨中软骨破坏中起关键作用。组织蛋白酶-K被用作破骨细胞活性的众所周知的标志物，因为这种酶主要来源于破骨细胞。NF-κ B配体受体激活因子（RANKL）在破骨细胞形成中起重要作用。虽然最近的一项研究表明RANKL参与牙周病的发病机制，但以前没有人检查过人类受试者体液中组织蛋白酶K的水平。如果组织蛋白酶-K和RANKL的存在可以在体液中检测到，这将是这些体液浸泡的组织中破骨细胞分化和/或活化的间接证据。该通讯报告了正常受试者和重度、中度和轻度疾病患者龈沟液（GCF）中组织蛋白酶-K和RANKL的体内浓度。在牙周炎患者的GCF中检测到组织蛋白酶-K和RANKL的浓度增加（与对照受试者相比P < 0.005）。组织蛋白酶-K与RANKL水平呈正相关（r = 0.726），提示二者均参与牙周病的骨破坏。(C)2007爱思唯尔有限公司保留所有权利。
Cathepsin-K is a highly expressed cysteine protease, and it plays a key role in bone remodeling and cartilage breakdown in bone. Cathepsin-K is used as a well-known marker of osteoclast activity, because this enzyme is mainly derived from osteoclasts. The receptor activator for NF-kappa B ligand (RANKL) plays an important role in osteoclast formation. Although a recent study suggests the involvement of RANKL in the pathogenesis of periodontal disease, no one has previously examined the level of cathepsin-K in the body fluid of human subjects. If the presence of cathepsin-K, as well as RANKL, can be detected in body fluids, it would be indirect proof of the differentiation and/or activation of osteoclasts in the tissues bathed by these fluids. This communication reports on the in vivo concentrations of cathepsin-K and RANKL in the gingival crevicular fluid (GCF) of normal subjects and those patients with severe, moderate, and mild forms of the disease. increased concentrations of cathepsin-K and RANKL were detected in the GCF from patients with periodontitis (P < 0.005 versus control subjects). Also, there was a positive correlation between cathepsin-K and RANKL levels (r = 0.726), suggesting that both of them contribute to osteoclastic bone destruction in periodontal disease. (C) 2007 Elsevier Ltd. All rights reserved.