The plasma membrane-bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana
The plasma membrane-bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana
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DOI：
10.1038/nbt949
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发表时间：
2004-04-01
影响因子：
46.9
通讯作者：
Wang, XM
中科院分区：
文献类型：
--
作者：
Li, WQ;Li, MY;Wang, XM
冻害是限制植物生产力和地理分布的主要环境因子。在这里，我们表明，冷冻耐受性可以操纵拟南芥的基因编码磷脂酶D δ（PLDdelta），这是参与膜脂水解和细胞信号转导的基因的遗传改变。基因敲除质膜相关的PLD δ使A.拟南芥植物对冷冻更敏感，而PLDdelta的过表达增加了耐冷冻性。脂质分析表明，PLD δ贡献了约20%的磷脂酸在野生型植物中产生的冷冻过程中，和PLD δ的过表达增加了磷脂酸的种类的生产。PLD δ的改变并不影响冷调节基因COR47或COR78的表达，也不改变冷诱导的脯氨酸或可溶性糖的增加，这表明PLD途径是对冷冻的响应的独特决定因素，并可能为提高植物的抗冻性提供机会。
Freezing injury is a major environmental limitation on the productivity and geographical distribution of plants. Here we show that freezing tolerance can be manipulated in Arabidopsis thaliana by genetic alteration of the gene encoding phospholipase Ddelta (PLDdelta), which is involved in membrane lipid hydrolysis and cell signaling. Genetic knockout of the plasma membrane-associated PLDdelta rendered A. thaliana plants more sensitive to freezing, whereas overexpression of PLDdelta increased freezing tolerance. Lipid profiling revealed that PLDdelta contributed approximately 20% of the phosphatidic acid produced in wild-type plants during freezing, and overexpression of PLDdelta increased the production of phosphatidic acid species. The PLDdelta alterations did not affect the expression of the cold-regulated genes COR47 or COR78 or alter cold-induced increases in proline or soluble sugars, suggesting that the PLD pathway is a unique determinant of the response to freezing and may present opportunities for improving plant freezing tolerance.