Characterization of Prohibitins in Male Reproductive System and their Expression under Oxidative Stress
Characterization of Prohibitins in Male Reproductive System and their Expression under Oxidative Stress
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男性生殖系统中抑制素的表征及其在氧化应激下的表达
DOI：
10.1016/j.juro.2015.10.179
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发表时间：
2016-04-01
影响因子：
6.6
通讯作者：
Li, Jian Y.
中科院分区：
文献类型：
--
作者：
Li, Yan;Wang, Hai Y.;Li, Jian Y.
目的：材料与方法：采用半定量聚合酶链反应、免疫印迹、免疫组织化学和间接免疫荧光等方法，研究Prohibitin家族成员Prohibitin 1和2在雄性大鼠生殖系统中的表达和定位及其在氧化应激反应中的作用。使用市售的丙二醛试剂盒评价氧化损伤。结果：Prohibitin 1和2在人的各种组织中广泛表达，在附睾中的表达明显增高。在人类睾丸和附睾中，它们定位于不同细胞类型的细胞质中。Prohibitin 1定位于人精子尾部的整个区域，而Prohibitin 2特异性定位于赤道区域。弱精子症患者精子中Prohibitin 2的阳性染色率和荧光强度均明显低于健康人。在大鼠生后发育过程中，睾丸和附睾中均一致表达阿比汀，提示其具有保守和重要的生物学功能。此外，在阿霉素注射诱导的氧化应激下，prohibitin 1和2的表达显着下调，在大鼠睾丸具有显着的组织形态学的changes.Conclusions：据我们所知，这项研究是第一次系统的研究，在男性生殖系统的抑制素。它为进一步的功能研究奠定了基础，并为氧化应激诱导的不孕症提供了潜在的治疗靶点。
Purpose: We investigated the expression and location of prohibitin 1 and 2 of the prohibitin family in the male reproductive system and their potential roles during the oxidative stress response in a rat model.Materials and Methods: Semiquantitative polymerase chain reaction, Western blot, immunohistochemistry and indirect immunofluorescence were performed to examine the expression and localization of prohibitins. Oxidative damage was evaluated using a commercially available malondialdehyde kit. Histological damage induced by doxorubicin injection was examined by hematoxylin and eosin staining.Results: Prohibitin 1 and 2 were ubiquitously expressed in various human tissues with distinct high expression in the epididymis. In the human testis and epididymis they were localized in the cytoplasm of diverse cell types. Prohibitin 1 was located on the entire tail region of human ejaculated spermatozoa while prohibitin 2 was specifically localized on the equatorial region. In spermatozoa from young men with asthenozoospermia the percent of spermatozoa with positive staining as well as the fluorescence intensity of prohibitin 2 was much lower than in the spermatozoa of healthy donors. Uniform expression of prohibitins in the testis and epididymis of the rat during postnatal development suggested conserved and vital biological functions. Moreover under oxidative stress induced by doxorubicin injection the expression of prohibitin 1 and 2 was significantly down-regulated in the rat testis with significant histomorphological changes.Conclusions: To our knowledge this research represents the first systematic study of prohibitins in the male reproductive system. It lays the foundation for further functional studies and provides potential therapeutic targets for infertility induced by oxidative stress.