Isolation and characterization of basement membrane collagen from human placental tissue. Evidence for the presence of two genetically distinct collagen chains.
Isolation and characterization of basement membrane collagen from human placental tissue. Evidence for the presence of two genetically distinct collagen chains.
复制标题
从人胎盘组织中分离和表征基底膜胶原。
DOI：
10.1021/bi00581a028
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
E. J. Miller
中科院分区：
文献类型：
--
作者：
T. Kresina;E. J. Miller
托马斯F. Kresina和Edward J.米勒 * 摘要：利用选择性沉淀程序和CM-纤维素上天然形式的胶原蛋白色谱法，从胎盘组织的有限胃蛋白酶消化物中分离出含有基底膜胶原蛋白分子的独特胶原蛋白组分。这种胶原蛋白的变性产物的表征揭示了两类组分的存在。酸性更强的组分（C '、C和50 K [1]）分别代表原C链的修饰形式、与源自分离的基底膜的有限胃蛋白酶消化物的α链大小组分几乎相同的胶原蛋白链以及由原C链或C链的蛋白水解产生的较小分子量组分的混合物。更基本的组件（80 K和50 K2）。基底膜可以被认为是专门的细胞外基质，其用于形成各种组织隔室之间的边界。在这种作用中，膜可以用作选择性渗透屏障以及支撑元件。因此，它们在维持组织完整性方面至关重要。早期对更大和更容易分离的基底膜如透镜囊、肾小球基底膜、Reichert膜和Descemet膜的化学组成的研究明确地确定，这些结构含有高比例的胶原序列分子，尽管其比例可变（Kefalides，1973）。然而，胶原成分的确切性质及其在基底膜内的分子组织尚未被很好地定义，并且是一些研究的主题。
Thomas F. Kresina and Edward J. Miller* abstract: A unique collagen fraction containing basement membrane collagen molecules has been isolated from limited pepsin digests of placental tissue utilizing selective precipitation procedures and chromatography of the collagen in native form on CM-cellulose. Characterization of the denaturation products of this collagen has revealedthe presence of two classes of components. The more acidic components (C', C, and 50K [) represent, respectively, a modified form of the pro-C chain, a collagen chain virtually identical with the a-chain-sized component derived from limited pepsin digests of isolated basement membranes, and a mixture of smaller molecular weight components arising from proteolysis of either the pro-C or C chain. The more basic components (80K and 50K2). Basement membranes may be regarded as specialized extracellular matrices which serve to form the boundaries between various tissue compartments. In this role, the membranes may function as selective permeability barriers as well as supporting elements. They are, therefore, of critical importance in the maintenance of tissue integrity. Early studies on the chemical composition of the larger and more readily isolated basement membranes such as the lens capsule, glomerular basement membrane, Reichert’s mem-brane, and Descemet’s membrane definitely established that such structurescontain a high, albeit variable, proportion of molecules with collagenous sequences (Kefalides, 1973). However, the precise nature of the collagenous constituents and their molecular organization within basement membranes have not been well-defined and are the subject of some