Effects of "Danzhi Decoction" on Chronic Pelvic Pain, Hemodynamics, and Proinflammatory Factors in the Murine Model of Sequelae of Pelvic Inflammatory Disease.
Effects of "Danzhi Decoction" on Chronic Pelvic Pain, Hemodynamics, and Proinflammatory Factors in the Murine Model of Sequelae of Pelvic Inflammatory Disease.
复制标题
“丹栀汤”对盆腔炎后遗症模型小鼠慢性盆腔疼痛、血流动力学及促炎因子的影响
DOI：
10.1155/2015/547251
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Jin Z
中科院分区：
文献类型：
--
作者：
Bu X;Liu Y;Lu Q;Jin Z
Objective.探讨丹栀汤对慢性盆腔炎性疾病后遗症（SPID）小鼠模型慢性盆腔痛（CPP）、血流动力学及促炎因子的影响。方法. SPID小鼠随机接受高剂量DZD、中剂量DZD、低剂量DZD、阿司匹林和溶媒治疗3个发情周期。采用小鼠鬼脸量表（MGS）评价CPP;采用上生殖道、盆壁和肠系膜血流动力学评价SPID小鼠的血流动力学;采用Western blot和免疫组化法检测血管内皮生长因子（VEGF）、血管生成素-2（Ang-2）和骨桥蛋白（OPN）的表达。结果剂量依赖性DZD治疗显著降低MGS评分，加速骨盆血流，并降低上生殖道VEGF，Ang-2和OPN的表达。结论和讨论。DZD能明显改善SPID小鼠盆腔瘀血微环境的血流动力学。CPP与盆腔瘀血微环境有关。此外，DZD可能在抗炎过程中发挥积极作用。
Objective. To evaluate the effect of Danzhi decoction (DZD) on chronic pelvic pain (CPP), hemodynamics, and proinflammatory factors of sequelae of pelvic inflammatory diseases (SPID) in murine model. Methods. SPID mice were randomly treated with high-dose DZD, mid-dose DZD, low-dose DZD, aspirin, and vehicle for 3 estrous circles. The Mouse Grimace Scale (MGS) was performed to evaluate CPP; blood flows of the upper genital tract, pelvic wall, and mesentery were used to assess hemodynamics in SPID mice; expressions of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2), and osteopontin (OPN) were measured by Western blot and immunochemistry. Results. Treatment with dose-dependent DZD significantly decreased the MGS scores, accelerated blood flows of the pelvis, and reduced expressions of VEGF, Ang-2, and OPN in the upper genital tract. Conclusions and Discussions. DZD was effective in relieving CPP and improving hemodynamics of the pelvic blood-stasis microenvironment in SPID mice. There was a relationship between CPP and the pelvic blood-stasis microenvironment. Furthermore, DZD might play a positive role in the anti-inflammatory process.