Comparison of polymerase chain reaction assay, bacteriologic culture, and serologic testing in assessment of prevalence of urinary shedding of leptospires in dogs
Comparison of polymerase chain reaction assay, bacteriologic culture, and serologic testing in assessment of prevalence of urinary shedding of leptospires in dogs
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DOI：
10.2460/javma.2003.222.1230
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发表时间：
2003-05-01
期刊：
影响因子：
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通讯作者：
Chengappa, MM
中科院分区：
文献类型：
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作者：
Harkin, KR;Roshto, YM;Chengappa, MM
方法：比较尿液样本的聚合酶链反应（PCR）检测、血清学检测和尿液细菌培养的结果，以确定犬尿中钩端螺旋体脱落的患病率。设计：系列病例研究。动物：500只犬，在不考虑健康状况的情况下进行系列评估。程序：通过PCR检测和尿液细菌培养检查尿液样本中的钩端螺旋体。对血清型犬，布拉迪斯拉发，波莫纳，黄疸型，流感伤寒，和hardjo.Results-Titers大于或等于1：100对至少1个血清型的抗体进行了分析血液样本中检测到104（20.8%）狗，和效价大于或等于1：400检测到41（8.2%）狗。高滴度检测最常见的血清型流感伤寒，其次是黄疸型，犬，波莫纳，布拉迪斯拉发，和hardjo。在14只狗中检测到> 1个血清型的高滴度。在41只（8.2%）犬中获得阳性PCR检测结果，其中仅9只犬的滴度大于或等于1：100。未从任何犬的尿液中培养出钩端螺旋体。只有4只狗有临床钩端螺旋体病。在6个月的评估期内，总体疾病患病率为0.8%。与PCR法相比，血清学法预测犬钩体脱落的敏感性为22%，特异性为79%，阳性预测值为9%，阴性预测值为92%。结论与临床意义血清学检测是尿脱落的不良预测因子。临床上正常的狗只如果带有钩端螺旋体，可能会对主人造成人畜共患病的危险。
Objective-To compare results of polymerase chain reaction (PCR) testing of urine samples, serologic testing, and bacteriologic culture of urine to determine prevalence of urinary shedding of leptospires in dogs.Design-Serial case study.Animals-500 dogs evaluated serially without regard to health status.Procedure-Urine samples were examined via PCR assay and bacteriologic culture for leptospires. Blood samples were analyzed for antibodies against serovars canicola, bratislava, pomona, icterohemorrhagiae, grippotyphosa, and hardjo.Results-Titers greater than or equal to 1:100 against at least 1 serovar were detected in 104 (20.8%) dogs, and titers greater than or equal to 1:400 were detected in 41 (8.2%) dogs. High titers were detected most commonly to serovar grippotyphosa, followed by icterohemorrhagiae, canicola, pomona, bratislava, and hardjo. High titers to > 1 serovar were detected in 14 dogs. A positive PCR assay result was obtained in 41 (8.2%) dogs, only 9 of which had a titer greater than or equal to 1:100. Leptospires were not cultured from the urine of any dog. Only 4 dogs had clinical leptospirosis. Overall disease prevalence was 0.8% for the 6-month evaluation period. Compared with PCR assay, serologic testing for predicting shedding had a sensitivity of 22%, specificity of 79%, positive predictive value of 9%, and negative predictive value of 92%.Conclusions and Clinical Relevance Irrespective of health status, 8.2% of dogs were shedding pathogenic leptospires. Serologic testing was a poor predictor of urinary shedding. Clinically normal dogs that shed leptospires may pose a zoonotic risk to their owners.