DETERMINATION OF SERUM CREATININE BY A DIRECT COLORIMETRIC METHOD

DETERMINATION OF SERUM CREATININE BY A DIRECT COLORIMETRIC METHOD
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DOI:
10.1016/0009-8981(73)90466-x
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发表时间:
1973-01-01
影响因子:
5
通讯作者:
TIDERSTR.G
TIDERSTR.G
中科院分区:
医学3区
文献类型:
--
作者:
HEINEGAR.D;TIDERSTR.G

文献摘要

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通过在反应混合物中加入十二烷基硫酸钠(SDS)和硼酸盐对Jaffe反应进行了改性。一种方法已经制定允许血清肌酐测定不事先去蛋白。在使用该试剂直接测定血清肌酐时,所得到的值与用色谱分离肌酐的方法所得到的值有很好的相关性。
The Jaffe reaction has been modified by the addition of sodium-dodecyl-sulfate (SDS) and borate to the reaction mixture. A method has been elaborated permitting serum creatinine determination without prior deproteinisation.On direct serum creatinine determination using this reagent, values are obtained that correlate well to those obtained with a method involving isolation of creatinine with a chromatographic procedure.