DIFFERENTIAL PROLIFERATION OF FIBROBLASTS CULTURED FROM NORMAL AND FIBROTIC HUMAN LUNGS
DIFFERENTIAL PROLIFERATION OF FIBROBLASTS CULTURED FROM NORMAL AND FIBROTIC HUMAN LUNGS
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DOI：
10.1164/ajrccm/138.3.703
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发表时间：
1988-09-01
期刊：
影响因子：
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通讯作者：
RABINOVITCH, PS
中科院分区：
文献类型：
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作者：
RAGHU, G;CHEN, YC;RABINOVITCH, PS
我们研究了培养的早期传代人肺成纤维细胞（HDPFC）在几种生长因子存在下生长时的细胞周期行为。在不同来源的培养物中研究了生长速率和生长组分。85 .+-。在含10%血清或生长因子的基础培养基中，从早期纤维化标本中培养的HDPFC细胞有5%（SE）的细胞处于周期状态。相比之下，55% .+-.正常肺的HDPFC仅为31% ± 0.8%（SE）。5%（SE）的HDPFC培养的标本与致密纤维化在这些培养条件下循环。通过从成分确定的培养基中去除五种生长因子中的任何一种获得的结果表明，改变的生长模式与检测的任何单一生长因子无关。这些研究与从早期纤维化标本中培养的HDPFC比晚期纤维化标本中培养的HDPEC具有更大的增殖潜力的假设一致。
We studied the behavior of the cell cycle of cultured, early-passage human diploid pulmonary fibroblastilke cells (HDPFC) when grown in the presence of several growth factors. The rates of growth and growth fractions were studied in cultures from different origins. As much as 85 .+-. 5% (SE) of HDPFC cultured from specimens with early fibrosis were cycling when the cells were grown in basal media supplemented wiht 10% serum or growth factors. In contrast, 55% .+-. 8% (SE) of HDPFC from normal lung and only 31% .+-. 5% (SE) of HDPFC cultured from specimens with dense fibrosis were cycling in those culture conditions. Results obtained by removal of any of the five growth factors from the defined medium indicated that the altered growth patterns were not related to any single growth factor tested. These studies are consistent with the hypothesis that HDPFC cultured from specimens with early fibrosis have a greater proliferative potential than HDPEC from late fibrosis.