Multi-angle lensless digital holography for depth resolved imaging on a chip.
Multi-angle lensless digital holography for depth resolved imaging on a chip.
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DOI：
10.1364/oe.18.009690
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发表时间：
2010-04-26
期刊：
影响因子：
3.8
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Su TW;Isikman SO;Bishara W;Tseng D;Erlinger A;Ozcan A
介绍了一种多角度无透镜全息成像平台，可以准确表征多层微通道内细胞的轴向和横向位置。在该平台上，采用部分相干照明，记录芯片上微物体在不同照明角度下的无透镜数字全息图。当光源的照明角度倾斜时，这些数字全息图开始在传感器平面上横向移动。由于可以精确地计算出每个物体全息图的横向位移的确切量，其精度超过光的衍射极限，因此可以在不使用任何透镜的情况下在大视场范围内确定每个细胞离基片的高度。我们演示了这个多角度无透镜成像平台的概念证明，通过使用发光二极管来表征位于芯片上的各种尺寸的微粒子，这些微粒子具有亚微米轴向和横向定位，视野范围超过~60 mm2。此外，我们成功地应用这种无透镜成像方法来同时表征位于多层微通道的血液样本，包括每层细胞的计数、个体厚度和体积。由于该平台不需要任何透镜、激光器或其他笨重的光学/机械组件，因此它为涉及芯片上实验室系统的细胞计数和诊断应用提供了一种紧凑、高通量的替代方法。
A multi-angle lensfree holographic imaging platform that can accurately characterize both the axial and lateral positions of cells located within multi-layered micro-channels is introduced. In this platform, lensfree digital holograms of the micro-objects on the chip are recorded at different illumination angles using partially coherent illumination. These digital holograms start to shift laterally on the sensor plane as the illumination angle of the source is tilted. Since the exact amount of this lateral shift of each object hologram can be calculated with an accuracy that beats the diffraction limit of light, the height of each cell from the substrate can be determined over a large field of view without the use of any lenses. We demonstrate the proof of concept of this multi-angle lensless imaging platform by using light emitting diodes to characterize various sized microparticles located on a chip with sub-micron axial and lateral localization over ~60 mm2 field of view. Furthermore, we successfully apply this lensless imaging approach to simultaneously characterize blood samples located at multi-layered micro-channels in terms of the counts, individual thicknesses and the volumes of the cells at each layer. Because this platform does not require any lenses, lasers or other bulky optical/mechanical components, it provides a compact and high-throughput alternative to conventional approaches for cytometry and diagnostics applications involving lab on a chip systems.