Application of laser scan microscopy in vivo for wound healing characterization
Application of laser scan microscopy in vivo for wound healing characterization
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DOI：
10.1002/lapl.201010038
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发表时间：
2010-06
影响因子：
1.7
通讯作者：
V. Czaika;A. Alborova;W. Sterry;J. Lademann;S. Koch
中科院分区：
文献类型：
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作者：
V. Czaika;A. Alborova;W. Sterry;J. Lademann;S. Koch
考虑到人口年龄的增长，伤口愈合障碍在临床常规中变得越来越重要。伤口愈合乳膏和洗剂的开发以及治疗控制需要对伤口愈合过程进行客观评价，这代表了屏障的破坏。因此，经常进行经表皮水分损失测量。这些测量具有的缺点是，它们受到位于慢性伤口表面上的间质液以及乳膏和洗剂的水组分的干扰。此外，TEWL测量对温度变化和志愿者的焦虑非常敏感。在本研究中，在体内激光扫描显微镜被用来分析的上皮再生和屏障恢复的标准化伤口所产生的吸引水泡技术。结果表明，这种非侵入性的在线光谱方法允许在没有任何干扰的情况下评估伤口愈合过程。发现伤口愈合不仅从伤口边缘开始，而且从毛囊开始。体内激光扫描显微镜非常适合于评估伤口愈合霜的疗效和治疗控制。
Considering the advancing age of the population, wound healing disturbances are becoming increasingly important in clinical routine. The development of wound healing creams and lotions as well as therapy control require an objective evaluation of the wound healing process, which represents the destruction of the barrier. Therefore, transepidermal water loss measurements are often carried out. These measurements have the disadvantage that they are disturbed by the interstitial fluid, which is located on the surface of chronic wounds and also by water components of the creams and lotions. Additionally, the TEWL measurements are very sensitive to temperature changes and to the anxiety of the volunteers. In the present study, in vivo laser scanning microscopy was used to analyze the reepithelialization and barrier recovery of standardized wounds produced by the suction blister technique. It was demonstrated that this non-invasive, on-line spectroscopic method allows the evaluation of the wound healing process, without any disturbances. It was found that the wound healing starts not only from the edges of the wound, but also out of the hair follicles. The in vivo laser scanning microscopy is well suited to evaluate the efficacy of wound healing creams and for therapy control.