Comparison of particulate guanylate cyclase in cells with and without atrial natriuretic peptide receptor binding activity.
Comparison of particulate guanylate cyclase in cells with and without atrial natriuretic peptide receptor binding activity.
复制标题
具有和不具有心钠素受体结合活性的细胞中颗粒鸟苷酸环化酶的比较。
DOI：
10.1007/bf00225217
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发表时间：
1989
影响因子：
4.3
通讯作者：
Murad,F
中科院分区：
文献类型：
--
作者：
Waldman,SA;Leitman,DC;Chang,LY;Murad,F
已经鉴定了一种不具有可测量的ANP结合但具有活性颗粒鸟苷酸环化酶的肾细胞系（PK）。这种酶的物理特性进行了比较与颗粒鸟苷酸环化酶和心钠素受体从大鼠肺分离。虽然受体和酶似乎存在于肺中的同一蛋白质上，而来自PK 11细胞的环化酶不具有ANP结合活性，但这些蛋白质表现出相同的物理特性。在凝胶过滤层析过程中，来自PK 1细胞和大鼠肺的鸟苷酸环化酶和来自肺的ANP受体共洗脱，Stokes半径为6.1 nm。此外，这些活动共迁移通过蔗糖密度梯度与S20，w值为10.4至10.9。使用这些参数，估计所有三种活性的分子量约为270 kD。此外，这些酶的活动表现出类似的迁移率在等电聚焦凝胶，与6.1的pI。因此，虽然来自肺的颗粒鸟苷酸环化酶可能具有受体结合活性，但它在物理上与这种酶的一种形式相同，没有可测量的结合活性。这些意见的可能解释进行了讨论。
A line of kidney cells (PK,) which does not possess measurable ANP binding but has an active particulate guanylate cyclase has been identified. The physical characteristics of this enzyme were compared with those of particulate guanylate cyclase and ANP receptors isolated from rat lung. Although receptor and enzyme appear to reside on the same protein in the lung while the cyclase from PK1cells does not possess ANP binding activity, these proteins exhibit identical physical characteristics. Guanylate cyclase from PK1cells and rat lung and ANP receptor from lung co-eluted during gel filtration chromatography, with a Stokes radius of 6.1 nm. Also, these activities co-migrated through sucrose density gradients with S20,wvalues of 10.4 to 10.9. Using these parameters, a molecular weight of about 270 kD was estimated for all three activities. Furthermore, these enzyme activities exhibited similar mobilities in isoelectric focusing gels, with a pI of 6.1. Thus, although particulate guanylate cyclase from lung presumably possesses receptor binding activity, it is physically identical to a form of this enzyme associated with no measurable binding activity. Possible explanations for these observations are discussed.