CyberKnife radiation therapy as a platform for translational mouse studies
CyberKnife radiation therapy as a platform for translational mouse studies
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射波刀放射治疗作为转化小鼠研究的平台
DOI：
10.1080/09553002.2021.1934749
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发表时间：
2021
影响因子：
2.6
通讯作者：
Herter JM
中科院分区：
文献类型：
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作者：
Kiljan M;Weil S;Vásquez-Torres A;Hettich M;Mayer M;Ibruli O;Reinscheid M;Heßelmann I;Niu LN;Sahbaz Y;Baues C;Baus WW;Kamp F;Marnitz S;Herter-Sprie GS;Herter JM
目的放射治疗（RT）是一种常见的非手术治疗癌症患者的管理。虽然基因工程小鼠模型（GEMM）重现人类疾病，但传统的线性粒子加速器系统不适合这些鼠肿瘤的最先进的图像引导靶向RT（IGRT）。我们采用CyberKnife（CK; Accuray）平台对GEMM衍生的非小细胞肺癌（NSCLC）病变进行IGRT。材料和方法使用CK RT平台照射GEMM衍生的KrasLSL-G12 D/+/Trp 53 fl/fl驱动的NSCLC侧腹肿瘤。我们应用IGRT的2，4，6，和8戈伊，使用5-12.5 mm的字段大小的平均总肿瘤体积（GTV）为0.9 cm 3，使用Xsight脊柱跟踪（Accuray）。我们观察到，与低剂量（2-4戈伊）相比，高RT剂量（6-8戈伊）在1小时内使肿瘤浸润性白细胞（TIL）的绝对细胞数减少约一半，但即使用低剂量RT（2戈伊），发现TIL在8- 10天后也减少。结论我们在此证明CK RT系统可以高精度地对小鼠肿瘤进行靶向IGRT，构成了一种新的有前途的方法。用于翻译小鼠RT研究的平台。
PurposeRadiation therapy (RT) is a common nonsurgical treatment in the management of patients with cancer. While genetically engineered mouse models (GEMM) recapitulate human disease, conventional linear particle accelerator systems are not suited for state-of-the-art, imageguided targeted RT (IGRT) of these murine tumors. We employed the CyberKnife (CK; Accuray) platform for IGRT of GEMM-derived non-small cell lung cancer (NSCLC) lesions.Material and methodsGEMM-derived KrasLSL-G12D/+/Trp53fl/fl-driven NSCLC flank tumors were irradiated using the CK RT platform. We applied IGRT of 2, 4, 6, and 8 Gy using field sizes of 5–12.5 mm to average gross tumor volumes (GTV) of 0.9 cm3 using Xsight Spine Tracking (Accuray).ResultsWe found that 0 mm planning target volume (PTV) margin is sufficient for IGRT of murine tumors using the CK. We observed that higher RT doses (6–8 Gy) decreased absolute cell numbers of tumor infiltrating leukocytes (TIL) by approximately half compared to low doses (2–4 Gy) within 1 h, but even with low dose RT (2 Gy) TIL were found to be reduced after 8–24 h.ConclusionWe here demonstrate that the CK RT system allows for targeted IGRT of murine tumors with high precision and constitutes a novel promising platform for translational mouse RT studies.
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影响因子：
5.3
作者：
Herter, Jan M.;Rossaint, Jan;Zarbock, Alexander
通讯作者：
Zarbock, Alexander
影响因子：
3.6
作者：
Tubin, Slavisa;Popper, Helmut H.;Brcic, Luka
通讯作者：
Brcic, Luka
影响因子：
28.2
作者：
Skoulidis F;Byers LA;Diao L;Papadimitrakopoulou VA;Tong P;Izzo J;Behrens C;Kadara H;Parra ER;Canales JR;Zhang J;Giri U;Gudikote J;Cortez MA;Yang C;Fan Y;Peyton M;Girard L;Coombes KR;Toniatti C;Heffernan TP;Choi M;Frampton GM;Miller V;Weinstein JN;Herbst RS;Wong KK;Zhang J;Sharma P;Mills GB;Hong WK;Minna JD;Allison JP;Futreal A;Wang J;Wistuba II;Heymach JV
通讯作者：
Heymach JV
影响因子：
50.3
作者：
Klug, Felix;Prakash, Hridayesh;Beckhove, Philipp
通讯作者：
Beckhove, Philipp
射波刀放射外科治疗脊柱肿瘤。
影响因子：
--
作者：
P. Gerszten;S. Burton;C. Ozhasoglu
通讯作者：
C. Ozhasoglu