Casein kinase II is required for proper cell division and acts as a negative regulator of centrosome duplication in Caenorhabditis elegans embryos.
Casein kinase II is required for proper cell division and acts as a negative regulator of centrosome duplication in Caenorhabditis elegans embryos.
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酪蛋白激酶II是适当细胞分裂所必需的，并且是秀丽隐杆线虫胚胎中的中心体重复的负调节剂。
DOI：
10.1242/bio.022418
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发表时间：
2017-01-15
期刊：
影响因子：
2.4
通讯作者：
Song MH
中科院分区：
文献类型：
--
作者：
Medley JC;Kabara MM;Stubenvoll MD;DeMeyer LE;Song MH
中心体是微管的主要组织中心，在细胞周期中协调微管动力学。正确的中心体数目对于建立双极有丝分裂纺锤体是至关重要的，从而确保染色体的精确分离。因此，中心粒必须在每个细胞周期复制一次，每个母中心粒复制一个子代，这一过程需要核心因子和调节剂之间的高度协调作用。蛋白质磷酸化被证明可以调节中心体蛋白的稳定性、定位和活性。本文报道了酪蛋白激酶II（CK 2）在秀丽隐杆线虫早期胚胎中的功能。CK 2的催化亚基（KIN-3/CK 2 α）定位于细胞核、中心体和中间体。CK 2失活导致细胞分裂缺陷，包括染色体错误分离、胞质分裂失败和异常中心体行为。此外，消耗或抑制CK 2的激酶活性会导致中心体的ZYG-1水平升高，从而恢复zyg-1突变体的中心体复制和胚胎活力。我们的数据表明，CK 2的功能在细胞分裂和负调控中心体复制的激酶依赖性的方式。总结：保守的蛋白激酶CK 2负调节中心体组装，并且是早期C.线虫胚胎
Centrosomes are the primary microtubule-organizing centers that orchestrate microtubule dynamics during the cell cycle. The correct number of centrosomes is pivotal for establishing bipolar mitotic spindles that ensure accurate segregation of chromosomes. Thus, centrioles must duplicate once per cell cycle, one daughter per mother centriole, the process of which requires highly coordinated actions among core factors and modulators. Protein phosphorylation is shown to regulate the stability, localization and activity of centrosome proteins. Here, we report the function of Casein kinase II (CK2) in early Caenorhabditis elegans embryos. The catalytic subunit (KIN-3/CK2α) of CK2 localizes to nuclei, centrosomes and midbodies. Inactivating CK2 leads to cell division defects, including chromosome missegregation, cytokinesis failure and aberrant centrosome behavior. Furthermore, depletion or inhibiting kinase activity of CK2 results in elevated ZYG-1 levels at centrosomes, restoring centrosome duplication and embryonic viability to zyg-1 mutants. Our data suggest that CK2 functions in cell division and negatively regulates centrosome duplication in a kinase-dependent manner. Summary: The conserved protein kinase CK2 negatively regulates centrosome assembly and is required for proper cell cycle progression and cytokinesis in early C. elegans embryos.