phd1+, a histone deacetylase gene of Schizosaccharomyces pombe, is required for the meiotic cell cycle and resistance to trichostatin A
phd1+, a histone deacetylase gene of Schizosaccharomyces pombe, is required for the meiotic cell cycle and resistance to trichostatin A
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DOI：
10.1016/s0014-5793(98)01124-7
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发表时间：
1998-10-02
期刊：
影响因子：
3.5
通讯作者：
Horinouchi, S
中科院分区：
文献类型：
--
作者：
Kim, YB;Honda, A;Horinouchi, S
从粟酒裂殖酵母中克隆了一个基因phd 1（+），该基因编码的蛋白与酵母和人类组蛋白脱乙酰酶（如酿酒酵母Rpd 3 p和人类HDAC 1）高度同源。从S.表达与FLAG表位融合的Phd 1的粟酒裂殖酵母细胞显示出组蛋白脱乙酰酶活性，其被组蛋白脱乙酰酶的特异性抑制剂--曲马斯他丁A（TSA）抑制。phd 1（+）的无效突变导致细胞总的组蛋白脱乙酰酶活性显著降低，对TSA的敏感性增加。虽然phd 1的破坏物在有丝分裂细胞周期和交配中没有明显缺陷，但在没有phd 1（+）的情况下，同宗配合的单倍体和异宗配合的二倍体细胞都不能形成孢子。这些结果表明，phd 1（+）编码组蛋白脱乙酰酶，这是参与减数分裂细胞周期的粟酒裂殖酵母。(C)1998年欧洲生物化学学会联合会。
A gene named phd1(+) encoding a protein highly homologous to the yeast and human histone deacetylases, such as Saccharomyces cerevisiae Rpd3p and human HDAC1, was cloned from Schizosaccharomyces pombe. The immune complex isolated from S. pombe cells expressing Phd1 fused to the FLAG epitope showed histone deacetylase activity, which was inhibited by trichostatin A (TSA), a specific inhibitor of histone deacetylase. The null mutation of phd1(+) resulted in a marked decrease in the total cellular histone deacetylase activity and an increase in the sensitivity to TSA, Although the phd1 disruptant showed no obvious defect in the mitotic cell cycle or mating, both homothallic haploid and heterothallic diploid cells failed to form spores in the absence of phd1(+). These results indicate that phd1(+) encodes a histone deacetylase, which is involved in the meiotic cell cycle in S, pombe. (C) 1998 Federation of European Biochemical Societies.