Piezoelectric actuator-based cell microstretch device with real-time imaging capability
Piezoelectric actuator-based cell microstretch device with real-time imaging capability
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DOI：
10.1063/1.4922220
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发表时间：
2015-06-01
期刊：
影响因子：
1.6
通讯作者：
Sato, Masaaki
中科院分区：
文献类型：
--
作者：
Deguchi, Shinji;Kudo, Shoko;Sato, Masaaki
细胞对物理拉伸的反应作为各种生理功能的调节器已被广泛研究。对于活细胞显微镜结合拉伸实验，细胞通常种植在可伸展的弹性体片上。在这种情况下，感兴趣细胞的位置往往在拉伸时移出视野，使得相同细胞的实时成像变得困难。为了避免这种情况，我们在这里描述了一种健壮的方法，其中这些细胞移位被最小化。细胞在定制设计的拉伸室中电镀，弹性体薄片覆盖一个小的细胞培养区域。细胞支撑室在倒置显微镜上通过使用压电致动器来拉伸，该致动器提供微小但精确控制的位移。即使在视野内的这种微小位移下，我们的设备也允许细胞经历生理上相关的拉伸水平。因此，在拉伸过程中可以连续观察到相同的细胞，从而潜在地使拉伸触发的快速动力学成像成为可能。(C)2015年作者(S)。
Cellular response to physical stretch has been extensively studied as a regulator of various physiological functions. For live cell microscopy combined with stretch experiments, cells are typically seeded on an extensible elastomer sheet. In this case, the position of the cells of interest tends to shift out of the field of view upon stretch, making real-time imaging of identical cells difficult. To circumvent this situation, here we describe a robust methodology in which these cell shifts are minimized. Cells are plated in a custom-designed stretch chamber with an elastomer sheet of a small cell culture area. The cell-supporting chamber is stretched on an inverted microscope by using a piezoelectric actuator that provides small, but precisely controlled displacements. Even under this small displacement within the filed of view, our device allows the cells to undergo physiologically relevant levels of stretch. Identical cells can thus be continuously observed during stretching, thereby potentially enabling imaging of stretch-triggered fast dynamics. (C) 2015 Author(s).