Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves
Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves
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DOI：
10.1104/pp.103.021022
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发表时间：
2003-07-01
期刊：
影响因子：
7.4
通讯作者：
Deng, XW
中科院分区：
文献类型：
--
作者：
Wu, P;Ma, LG;Deng, XW
在从生长培养基中去除 Pi 后的 3 天内，检查了拟南芥基因组表达模式对磷酸盐 (Pi) 饥饿的响应变化。磷饥饿后的前24小时，根部有效磷浓度下降了约22%，随后在磷饥饿后的第2和第3天缓慢恢复，但在磷饥饿的3天内，叶片中没有观察到显着变化。微阵列分析显示，在 Pi 饥饿开始后 72 小时内，阵列中存在的 6,172 个基因中有超过 1,800 个基因被调节了 2 倍或更多。对这些 Pi 饥饿反应基因的分析表明，它们属于广泛的功能类别。许多光合作用和氮同化基因被下调。 Pi 饥饿期间似乎会发生一系列复杂的代谢适应。转录因子和细胞信号蛋白的 100 多个基因因 Pi 饥饿而受到调节，这意味着细胞生长和发育的重大调节变化。这些调控基因中的很大一部分在响应 Pi 饥饿时在叶和根中表现出不同甚至对比的表达，支持了不同的植物器官针对生长介质中 Pi 短缺而使用不同的 Pi 饥饿响应策略的观点。
Arabidopsis genome expression pattern changes in response to phosphate (Pi) starvation were examined during a 3-d period after removal of Pi from the growth medium. Available Pi concentration was decreased after the first 24 h of Pi starvation in roots by about 22%, followed by a slow recovery during the 2nd and 3rd d after Pi starvation, but no significant change was observed in leaves within the 3 d of Pi starvation. Microarray analysis revealed that more than 1,800 of the 6,172 genes present in the array were regulated by 2-fold or more within 72 h from the onset of Pi starvation. Analysis of these Pi starvation-responsive genes shows that they belong to wide range of functional categories. Many genes for photosynthesis and nitrogen assimilation were down-regulated. A complex set of metabolic adaptations appears to occur during Pi starvation. More than 100 genes each for transcription factors and cell-signaling proteins were regulated in response to Pi starvation, implying major regulatory changes in cellular growth and development. A significant fraction of those regulatory genes exhibited distinct or even contrasting expression in leaves and roots in response to Pi starvation, supporting the idea that distinct Pi starvation response strategies are used for different plant organs in response to a shortage of Pi in the growth medium.