AMPEROMETRIC L-LACTATE-SENSING ELECTRODE BASED ON A POLYION COMPLEX LAYER CONTAINING LACTATE OXIDASE - APPLICATION TO SERUM AND MILK SAMPLES
AMPEROMETRIC L-LACTATE-SENSING ELECTRODE BASED ON A POLYION COMPLEX LAYER CONTAINING LACTATE OXIDASE - APPLICATION TO SERUM AND MILK SAMPLES
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DOI：
10.1016/0003-2670(95)00278-8
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发表时间：
1995-10-20
影响因子：
6.2
通讯作者：
HIRATA, Y
中科院分区：
文献类型：
--
作者：
MIZUTANI, F;YABUKI, S;HIRATA, Y
将乳酸氧化酶（LOD）固定在聚离子复合膜上，制备了L-乳酸安培酶电极。将含有聚-L-赖氨酸和LOD的水溶液置于玻碳电极上，并将聚（4-苯乙烯磺酸盐）的水溶液加入到聚阳离子/LOD混合物中并干燥。该酶电极的阳极电流（1V vs. Ag/AgCl）在加入L-乳酸后立即增加，响应时间<5s。线性响应L-乳酸观察到高达0.3 mM的检测限为0.1 μ M。将该电极应用于血清和酸奶中L-乳酸的测定：聚离子复合膜对L-乳酸具有选择性透过，截留分子量约为1000。100，其对L-抗坏血酸、尿酸和对乙酰氨基酚的电化学干扰有很好的抑制作用。电极可使用至少两个月。
An amperometric enzyme electrode for L-lactic acid was prepared by immobilizing lactate oxidase (LOD) in a polyion complex membrane. An aqueous solution containing poly-L-lysine and LOD was placed on a glassy carbon electrode, and an aqueous solution of poly(4-styrenesulfonate) was added to the polycation/LOD mixture and dried. The anodic current (at 1 V vs. Ag/AgCl) of this enzyme electrode increased immediately after the addition of L-lactic acid and the response time was < 5 s. A linear response to L-lactic acid was observed up to 0.3 mM with a detection limit of 0.1 mu M. The enzyme electrode was applied to the determination of L-lactic acid in sera and sour milk: the polyion complex membrane showed permselectivity based on the solute size with the molecular weight cut-off of ca. 100, which was very effective in suppressing the electrochemical interference by L-ascorbic acid, uric acid and acetaminophen. The electrode could be used for at least two months.