The transcriptome of the salivary glands of the female western black-legged tick Ixodes pacificus (Acari:Ixodidae)
The transcriptome of the salivary glands of the female western black-legged tick Ixodes pacificus (Acari:Ixodidae)
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DOI：
10.1016/j.ibmb.2005.05.007
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发表时间：
2005-10-01
影响因子：
3.8
通讯作者：
Ribeiro, JMC
中科院分区：
文献类型：
--
作者：
Francischetti, IMB;Pham, VM;Ribeiro, JMC
对太平洋硬蜱唾液腺cDNA文库进行测序，共获得1068条序列，平均未知核苷酸为1.9%，平均长度为487个碱基对。表达的序列标签的组装产生了557个重叠群，其中138个似乎编码基于推定的信号肽的翻译的分泌肽或蛋白质。基于BLASTX相似性的这些重叠群的66个匹配的肩突罗德肽序列，只有58%的序列同一性被发现，表明唾液蛋白的快速分歧，如先前观察到的蚊子和锥蝽属昆虫唾液蛋白。在这里，我们报告106大部分全长序列，聚集在16个不同的家庭：基本尾蛋白富含赖氨酸的羧基末端，Kunitz含蛋白质（monolaris，ixolaris和penthalaris家庭），脯氨酸丰富的肽，5-，9.4-和18.7-kDa的蛋白质的未知功能，除了金属蛋白酶（类PIII样）类似replysins。我们还发现了一个名为ixodegrins的去整合素家族，其显示出与variabilin的同源性，variabilin是来自蜱Dermacentor variabilis的GPIIb/IIIa拮抗剂。此外，我们还描述了与ADAMST-4（聚集蛋白聚糖酶）富含半胱氨酸结构域具有显著相似性的肽（此处命名为ixostatins）。许多分子被分配在脂质运载蛋白家族（组胺结合蛋白）;其他分子似乎参与氧化剂代谢，还有一些分子类似于ixodid蛋白，如抗补体ISAC。我们还首次鉴定了一种具有GGY重复序列的神经肽样蛋白（nlp-31），该蛋白可能具有抗菌活性。此外，16个新的蛋白质没有显着的相似性，其他蜱蛋白和37管家蛋白，可能是有用的系统发育研究进行了描述。这些蛋白质中的一些可用于研究血管生物学或免疫系统，用于疫苗开发，或作为免疫试剂检测先前暴露于蜱。电子版的手稿可以在http://www.ncbi.nlm.nih.gov/projects/omes/上找到。爱思唯尔有限公司出版
Sequencing of an Ixodes pacificus salivary gland cDNA library yielded 1068 sequences with an average undetermined nucleotide of 1.9% and an average length of 487 base pairs. Assembly of the expressed sequence tags yielded 557 contigs, 138 of which appear to code for secreted peptides or proteins based on translation of a putative signal peptide. Based on the BLASTX similarity of these contigs to 66 matches of Rodes scapularis peptide sequences, only 58% sequence identity was found, indicating a rapid divergence of salivary proteins as observed previously for mosquito and triatomine bug salivary proteins. Here we report 106 mostly full-length sequences that clustered in 16 different families: Basic-tail proteins rich in lysine in the carboxy-terminal, Kunitz-containing proteins (monolaris, ixolaris and penthalaris families), proline-rich peptides, 5-, 9.4- and 18.7-kDa proteins of unknown functions, in addition to metalloproteases (class PIII-like) similar to reprolysins. We also have found a family of disintegrins, named ixodegrins that display homology to variabilin, a GPIIb/IIIa antagonist from the tick Dermacentor variabilis. In addition, we describe peptides (here named ixostatins) that display remarkable similarities to the cysteine-rich domain of ADAMST-4 (aggrecanase). Many molecules were assigned in the lipocalin family (histamine-binding proteins); others appear to be involved in oxidant metabolism, and still others were similar to ixodid proteins such as the anticomplement ISAC. We also identified for the first time a neuropeptide-like protein (nlp-31) with GGY repeats that may have antimicrobial activity. In addition, 16 novel proteins without significant similarities to other tick proteins and 37 housekeeping proteins that may be useful for phylogenetic studies are described. Some of these proteins may be useful for studying vascular biology or the immune system, for vaccine development, or as immunoreagents to detect prior exposure to ticks. Electronic version of the manuscript can be found at http://www.ncbi.nlm.nih.gov/projects/omes/. Published by Elsevier Ltd.