SELECTIVE DESTRUCTION OF PROTEIN FUNCTION BY CHROMOPHORE-ASSISTED LASER INACTIVATION
SELECTIVE DESTRUCTION OF PROTEIN FUNCTION BY CHROMOPHORE-ASSISTED LASER INACTIVATION
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DOI：
10.1073/pnas.85.15.5454
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发表时间：
1988-08-01
影响因子：
11.1
通讯作者：
JAY, DG
中科院分区：
文献类型：
--
作者：
JAY, DG
生色团辅助激光灭活蛋白质的功能已经实现。在蛋白质与孔雀石绿(C.I.42000)结合的特定配体或抗体结合后，在染料吸收但不被细胞成分显著吸收的波长的激光照射下，它被选择性地灭活。溶液中和细胞表面的配基结合蛋白可以变性，而同一样品中的其他蛋白不会受到影响。发色团辅助激光灭活可以通过灭活活细胞上单个蛋白质的功能来研究细胞表面现象，这是细胞激光消融的分子延伸。与遗传学和特定抑制剂的使用相比，它具有优势，因为生物体内单个细胞的蛋白质功能可以通过聚焦激光来灭活。
Chromophore-assisted laser inactivation of protein function has been achieved. After a protein binds a specific ligand or antibody conjugated with malachite green (C.I. 42,000), it is selectively inactivated by laser irradiation at a wavelength of light absorbed by the dye but not significantly absorbed by cellular components. Ligand-bound proteins in solution and on the surfaces of cells can be denatured without other proteins in the same samples being affected. Chromopore-assisted laser inactivation can be used to study cell surface phenomena by inactivating the functions of single proteins on living cells, a molecular extension of cellular laser ablation. It has an advantage over genetics and the use of specific inhibitors in that the protein function of a single cell within the organism can be inactivated by focusing the laser beam.