Distinct cortical migrations from the medial and lateral ganglionic eminences.
Distinct cortical migrations from the medial and lateral ganglionic eminences.
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发表时间：
2001-02
期刊：
影响因子：
4.6
通讯作者：
S. Anderson;O. Marín;Carrie Horn;Kelly Jennings;J. Rubenstein
中科院分区：
文献类型：
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作者：
S. Anderson;O. Marín;Carrie Horn;Kelly Jennings;J. Rubenstein
最近的证据表明，大脑皮层的投射神经元和中间神经元通常源自不同的增殖区。皮质投射神经元起源于皮质心室区（VZ），然后径向迁移到皮质套，而大多数皮质中间神经元起源于基底端脑并切向迁移到发育中的皮质。先前使用标记增殖细胞和有丝分裂后细胞的方法进行的研究发现，皮质中间神经元从发育中的基底端脑的两个主要部分迁移：内侧和外侧神经节隆起（MGE 和 LGE）。由于这些研究标记细胞的方法不能区分增殖细胞和可能起源于其他地方的细胞，因此我们使用命运图谱和遗传方法研究了 MGE 和 LGE 对皮质中间神经元的贡献。将 BrdU 标记的 MGE 或 LGE 神经上皮移植到未标记的端脑切片的基底端脑中，使我们能够追踪源自这些原基的神经元的命运。我们已经确定，在神经发生早期，来自 MGE 的表达 GABA 的细胞主要通过中间区切向迁移到大脑皮层，而来自 LGE 的细胞则不然。在神经发生的后期，LGE 衍生的细胞也迁移到皮质中，尽管这种迁移主要通过室下区发生。其中一些 LGE 衍生细胞侵入皮质板并表达 GABA，而其他细胞则保留在皮质增殖区内，并似乎在妊娠后期变得有丝分裂活跃。此外，通过比较对 MGE 和 LGE 迁移具有不同影响的小鼠突变体的表型，我们提供了 MGE 和 LGE 可能产生不同亚型的皮质中间神经元的证据。
Recent evidence suggests that projection neurons and interneurons of the cerebral cortex are generally derived from distinct proliferative zones. Cortical projection neurons originate from the cortical ventricular zone (VZ), and then migrate radially into the cortical mantle, whereas most cortical interneurons originate from the basal telencephalon and migrate tangentially into the developing cortex. Previous studies using methods that label both proliferative and postmitotic cells have found that cortical interneurons migrate from two major subdivisions of the developing basal telencephalon: the medial and lateral ganglionic eminences (MGE and LGE). Since these studies labeled cells by methods that do not distinguish between the proliferating cells and those that may have originated elsewhere, we have studied the contribution of the MGE and LGE to cortical interneurons using fate mapping and genetic methods. Transplantation of BrdU-labeled MGE or LGE neuroepithelium into the basal telencephalon of unlabeled telencephalic slices enabled us to follow the fate of neurons derived from each of these primordia. We have determined that early in neurogenesis GABA-expressing cells from the MGE tangentially migrate into the cerebral cortex, primarily via the intermediate zone, whereas cells from the LGE do not. Later in neurogenesis, LGE-derived cells also migrate into the cortex, although this migration occurs primarily through the subventricular zone. Some of these LGE-derived cells invade the cortical plate and express GABA, while others remain within the cortical proliferative zone and appear to become mitotically active late in gestation. In addition, by comparing the phenotypes of mouse mutants with differential effects on MGE and LGE migration, we provide evidence that the MGE and LGE may give rise to different subtypes of cortical interneurons.