Partition Behavior of Bovine Serum Albumin in PEG2000‐Sodium Citrate‐Water Based Aqueous Two‐Phase System
Partition Behavior of Bovine Serum Albumin in PEG2000‐Sodium Citrate‐Water Based Aqueous Two‐Phase System
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DOI：
10.1080/15363830701313404
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发表时间：
2007-06
影响因子：
2.8
通讯作者：
M. Perumalsamy;T. Murugesan
中科院分区：
文献类型：
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作者：
M. Perumalsamy;T. Murugesan
摘要在25°、35°和45°C下，研究了成相组分、pH值、中性盐（NaCl）加入量对PEG 2000-柠檬酸钠双水相体系中牛血清白蛋白（BSA）分配的影响。通过在pH为5.0、6.0、7.0、8.0和9.0下改变相形成组分的浓度，用0.05 M、0.1 M、1.5 M、0.2 M、0.3 M和0.4 M的不同NaCl盐浓度来制备系统。由于两相系统中三价与二价柠檬酸盐离子的比例增加，BSA对下相的亲和力随着pH值的增加而增加。结果表明，BSA的分配依赖于化合物的相对疏水性以及它们的电荷。确定了牛血清白蛋白分配的最佳pH值和NaCl浓度。
Abstract The effect of phase forming components, pH, neutral salt (NaCl) addition on partitioning of bovine serum albumin (BSA) in PEG2000‐sodium citrate based aqueous two‐phase system was investigated at 25°, 35°, and 45°C. The systems were prepared by varying the phase forming components concentration at a pH of 5.0, 6.0, 7.0, 8.0, and 9.0 with different NaCl salt concentrations of 0.05 M, 0.1 M, 1.5 M, 0.2 M, 0.3 M, and 0.4 M. The affinity of the BSA for the lower phase increases with increase in pH, due to increase in ratio of trivalent to divalent citrate ions in the two‐phase system. It was confirmed that the partition of BSA depends on the relative hydrophobicity of the compounds as well as their charge. Optimum level of pH and NaCl concentration for the partitioning of bovine serum albumin was determined.