Endothelial cells influence the osteogenic potential of bone marrow stromal cells.
Endothelial cells influence the osteogenic potential of bone marrow stromal cells.
复制标题
DOI：
10.1186/1475-925x-8-34
复制
发表时间：
2009-11-17
影响因子：
3.9
通讯作者：
Mustafa K
中科院分区：
文献类型：
--
作者：
Xue Y;Xing Z;Hellem S;Arvidson K;Mustafa K
对骨细胞和内皮细胞在成骨过程中相互作用的深入了解有助于骨组织工程新策略的发展。本研究的目的是确定骨髓基质细胞（MSC）和人脐静脉内皮细胞（EC）之间的直接通信是否会影响MSC在无成骨因子培养基中的成骨潜能。在直接接触系统中加入EC后，用WST试验和免疫染色研究细胞活力和形态。采用Superarray法系统检测EC对MSC成骨分化的影响，并采用实时荧光定量PCR法进行验证。在以1：5（EC/MSC）的比例添加EC至MSC五天后，检测到细胞增殖和两种细胞类型之间的细胞桥的显著增加，以及碱性磷酸酶（ALP）的mRNA表达的增加。这种作用大于在培养基中加入成骨因子如地塞米松、抗坏血酸和β-甘油磷酸盐所观察到的作用。在成骨刺激培养基中培养的MSC中，转录因子Runx 2的表达增强，但不受EC诱导的影响。I型胶原的表达不受EC的影响，但在无成骨因子的培养基中生长的细胞比在成骨刺激培养基中生长的细胞表现出更高的表达。这些结果表明，EC和MSC共培养5天影响MSC的成骨分化，这种作用可能不依赖于Runx 2，并增强MSC的ALP产生。
Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential of MSC in osteogenic factor-free medium. After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. The effects on osteogenic differentiation of adding EC to MSC was systematically tested by the using Superarray assay and results were confirmed with real-time PCR. Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC) significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP). This effect was greater than that seen with addition of osteogenic factors such as dexamethasone, ascorbic acid and β-glycerophosphate to the culture medium. The expression of transcription factor Runx2 was enhanced in MSC incubated with osteogenic stimulatory medium, but was not influenced by induction with EC. The expression of Collagen type I was not influenced by EC but the cells grown in the osteogenic factor-free medium exhibited higher expression than those cultured with osteogenic stimulatory medium. These results show that co-culturing of EC and MSC for 5 days influences osteogenic differentiation of MSC, an effect that might be independent of Runx2, and enhances the production of ALP by MSC.