STEROID-RECEPTOR DISTRIBUTION IN 47892 BREAST CANCERS - A COLLABORATIVE STUDY OF 7 EUROPEAN LABORATORIES
STEROID-RECEPTOR DISTRIBUTION IN 47892 BREAST CANCERS - A COLLABORATIVE STUDY OF 7 EUROPEAN LABORATORIES
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DOI：
10.1016/0959-8049(94)00496-r
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发表时间：
1995-01-01
影响因子：
8.4
通讯作者：
MAGDELENAT, H
中科院分区：
文献类型：
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作者：
ROMAIN, S;BIDRON, CL;MAGDELENAT, H
EORTC受体研究组的七个实验室报告了乳腺癌细胞质中常规检测的雌激素（ER）和孕激素受体（PR）的分布。实验室间变异性较低，证明了中位数，并为阳性肿瘤的频率，通过酶免疫测定法（EIA）测量。如通过放射性配体结合试验（RLA）测量的，发现阳性肿瘤的频率存在较大的变化，这可能是由于截止水平和试验灵敏度的差异。随时间变化的分析清楚地表明，与RLA相比，ER-EIA值最初增加。变化的一个可能来源可能是ER-EIA试剂盒中的校准漂移。总之，类固醇受体的质量评估应通过比较每个实验室常规获得的共同标准和分布进行监测。随着时间的推移，内部分析对试剂调查也至关重要。
Seven laboratories of the EORTC Receptor Study Group reported the distribution of oestrogen (ER) and progesterone receptors (PR) routinely assayed in breast cancer cytosols. A low interlaboratory variability was demonstrated for the median values, and for the frequency of positive tumours as measured by enzyme immunoassay (EIA). Larger variations were found for the frequency of positive tumours, as measured by radioligand binding assay (RLA), They are probably due to differences in the cut-off levels and in the sensitivity of the assay. Analysis of the variability over time clearly demonstrated that the ER-EIA values initially increased compared with RLA. A possible source of variations could be the calibration drift in the ER-EIA kit. In conclusion, quality assessment of steroid receptors should be monitored by comparison of both common standards and distributions routinely obtained in each laboratory. In-house analysis over time is also essential for reagent survey.