Dynamic patterns of expression of BMP isoforms 2, 4, 5, 6, and 7 during chicken heart development
Dynamic patterns of expression of BMP isoforms 2, 4, 5, 6, and 7 during chicken heart development
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DOI：
10.1002/ar.a.20031
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发表时间：
2004-07-01
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影响因子：
--
通讯作者：
Van Den Hoff, MJB
中科院分区：
文献类型：
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作者：
Somi, S;Buffing, AAM;Van Den Hoff, MJB
骨形态发生蛋白(BMPs)在心脏发育中起重要作用。通过体外外植体分析，我们发现BMP对于心肌的形成是至关重要的。为了确定哪些BMP可能参与了在体心内外间充质的心肌形成，用原位杂交的方法研究了BMP2、-4、-5、-6和-7mRNA在HH5~44鸡胚心中的表达模式。在中胚层细胞向流出道心肌(HH10-23)募集的过程中，BMP2、-4、-5和-7mRNA在心肌远端边缘和侧翼间充质表达。完成后，BMP2和-4mRNA仅限于间质，BMP5和-7mRNA仅限于心肌。在静脉极，BMP2、-5和-7mRNA在下腔静脉远端心肌边缘表达，而BMP2、-5、-6和-7mRNA在肺静脉周围远端心肌表达。BMP4基因在两侧相邻间充质中均有表达。在房室垫和三尖瓣的肌化过程中，突入间充质的心肌细胞表达BMP2、-4、-5和-7mRNA，而Bmp6mRNA在垫间充质表达。BMP4、-5、-7mRNA表达于间充质近端出口间隔，BMP2、-6mRNA表达于间充质。这些BMPs在心脏远端和心脏内与心肌形成相关的时空表达模式提示BMPs在心肌形成中发挥作用。在瓣膜分层过程中，BMP4和-6mRNA在二尖瓣形成时的室侧表达，BMP4在三尖瓣形成时的室侧表达，BMP2、-4和-6mRNA在半月瓣形成时的血管侧表达。(C)2004年Wiley-Liss公司
Bone morphogentic proteins (BMPs) play an important role in cardiac development. Using an in vitro explant analysis, we show that BMPs are crucial for myocardium formation. As a first approach to identify which BMP may be involved in myocardium formation in intra- and extracardiac mesenchyme in vivo, a survey of the expression patterns of BMP2, -4, -5, -6, and -7 mRNA is prepared by in situ hybridization in chicken embryonic hearts from HH5 to 44. During recruitment of mesodermal cells to the outflow tract myocardium (HH10-23), BMP2, -4, -5, and -7 mRNA are expressed in the distal myocardial border and the flanking mesenchyme. After completion, BMP2 and -4 mRNA become restricted to the mesenchyme and BMP5 and -7 mRNA to the myocardium. At the venous pole, BMP2, -5, and -7 mRNA are expressed in the distal myocardial border of the caval vein, while BMP2, -5, -6, and -7 mRNA are expressed in the distal myocardium around the pulmonary vein. BMP4 mRNA is expressed in the adjacent mesenchyme at both sides. During muscularization of the atrioventricular cushions and the tricuspid valve, the cardiomyocytes that protrude into the mesenchyme express BMP2, -4, -5, and -7 mRNA, whereas BMP6 mRNA is expressed in the cushion mesenchyme. The myocardial protrusions formed in the mesenchymal proximal outlet septum express BMP4, -5, and -7 mRNA, while BMP2 and -6 mRNA are expressed in the mesenchyme. The spatiotemporal expression patterns of these BMPs in relation to myocardium formation at the distal ends and within the heart suggest a role for BMPs in myocardium formation. During delamination of the valves, BMP4 and -6 mRNA are expressed at the ventricular side of the forming mitral valve, BMP4 mRNA at the ventricular side of the forming tricuspid valve, and BMP2, -4, and -6 mRNA at the vascular side of the forming semilunar valves. (C) 2004 Wiley-Liss, Inc.