Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004
Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004
复制标题
DOI：
10.1128/jvi.79.16.10821-10825.2005
复制
发表时间：
2005-08-01
影响因子：
5.4
通讯作者：
Webster, RG
中科院分区：
文献类型：
--
作者：
Choi, YK;Nguyen, TD;Webster, RG
为了确定越南与人类感染相关的H5N1禽流感病毒是否已传播给猪，我们调查了2004年越南猪暴露于H5N1流感病毒的血清学证据。经病毒中和试验和蛋白质印迹分析，在检测的3，175份猪血清中，8份（0.25%）对2004年分离的禽H5N1流感病毒呈阳性。对2004年亚洲H5N1病毒在猪体内复制和传播的实验研究表明，所有测试的病毒都在猪的呼吸道中复制，但没有一种病毒传播给接触猪。鼻拭子的病毒滴度在第2天达到峰值，在4头试验猪中的2头的肝脏中检测到低滴度。我们的研究结果表明，猪可以感染高致命性的亚洲H5N1病毒，但这些病毒在实验条件下不易在猪之间传播。
To determine whether avian H5N1 influenza viruses associated with human infections in Vietnam had transmitted to pigs, we investigated serologic evidence of exposure to H5N1 influenza virus in Vietnamese pigs in 2004. Of the 3,175 pig sera tested, 8 (0.25%) were positive for avian H5N1 influenza viruses isolated in 2004 by virus neutralization assay and Western blot analysis. Experimental studies of replication and transmissibility of the 2004 Asian H5N1 viruses in pigs revealed that all viruses tested replicated in the swine respiratory tract but none were transmitted to contact pigs. Virus titers from nasal swabs peaked on day 2, and low titers were detected in the liver of two of the four pigs tested. Our findings indicate that pigs can be infected with highly lethal Asian H5N1 viruses but that these viruses are not readily transmitted between pigs under experimental conditions.