Regulation of dopamine-1A (D1A) receptor gene transcription.
Regulation of dopamine-1A (D1A) receptor gene transcription.
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DOI：
10.3109/10641969709080800
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发表时间：
1997
影响因子：
12.3
通讯作者：
D. Healy;D. A. O'Rourke
中科院分区：
文献类型：
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作者：
D. Healy;D. A. O'Rourke
D1 A受体主要在大脑和肾脏中表达。D1 A受体基因已经从人类、大鼠和猪中克隆出来，并且在每个物种中的组织相似。D1 A受体基因的5'侧翼区GC含量高，无TATA盒，含有多个Sp1结合位点。每个基因的5'侧翼和5'非翻译区内的核苷酸序列的比较和比对表明，最高序列同一性在转录起始位点上游约100个碱基的区域中。在5'侧翼区有许多转录因子的结合位点，包括Sp1和AP-2。上游大约200个碱基是保守的cAMP调节元件样序列。每个基因中某些顺式作用元件的保守位置表明D1 A受体基因调控表达的基本元件包含在5'侧翼区的前300个碱基内。
The D1A receptor is expressed primarily in the brain and kidney. The D1A receptor gene has been cloned from human, rat and pig and is organized similarly in each species. The 5' flanking region of the D1A receptor gene is high in GC content, is TATA box-less and contains multiple Sp1 binding sites. Comparison and alignment of the nucleotide sequences within the 5' flanking and 5' untranslated regions of each gene indicates that the highest sequence identity is in the area centered approximately 100 bases upstream from the transcription start site. There are numerous binding sites for transcription factors, including Sp1 and AP-2, in the 5' flanking region. Approximately 200 bases upstream is a conserved cAMP regulatory element-like sequence. The conserved position of certain cis-acting elements in each gene suggests that the essential elements for regulated expression of the D1A receptor gene are contained within the first 300 bases of the 5' flanking region.