Sensitive detection of glucose in human serum with oligonucleotide modified gold nanoparticles by using dynamic light scattering technique
Sensitive detection of glucose in human serum with oligonucleotide modified gold nanoparticles by using dynamic light scattering technique
复制标题
动态光散射技术用寡核苷酸修饰金纳米粒子灵敏检测人血清中的葡萄糖
DOI：
10.1016/j.bios.2012.09.015
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发表时间：
2013-03-15
影响因子：
12.6
通讯作者：
Shuai, Xintao
中科院分区：
文献类型：
--
作者：
Miao, Xiangmin;Ling, Liansheng;Shuai, Xintao
利用寡核苷酸功能化的金纳米粒子（Oligo-AuNPs）研制了动态光散射葡萄糖传感器。寡核苷酸5 '-SH-（A）（12）-AGACAAGAGAGG-3'（Oligo 1）修饰的AuNPs和寡核苷酸5 '-CAACAGAGAACG-（A）（12）-HS-3'（Oligo 2）修饰的AuNPs可以与寡核苷酸5 '-CGTTCTCTGTTGCCTCTTGTCT-3'（Oligo 3）杂交，这导致Oligo-AuNPs探针聚集，并引发其平均直径增加。而Oligo 3可被葡萄糖、葡萄糖氧化酶（GOD）和Fe 2+的混合物切割成DNA片段，且DNA片段不能与Oligo-AuNPs探针杂交。在3.7 nM Oligo 1-AuNPs、3.7 nM Oligo 2-AuNPs、8.0 μ g/mL GOD、100 nM Oligo 3和900 nM Fe 2+的条件下，Oligo-AuNPs探针的平均直径随着葡萄糖浓度的增加在50 pmol/L至5.0 nmol/L的范围内线性减小，检测限为38 pmol/L（3 sigma/slope）。此外，五种糖对Oligo-AuNPs探针、GOD和Fe 2+的混合物的平均直径没有影响，这证明了测定的良好选择性。(C)2012爱思唯尔有限公司版权所有。
Dynamic light scattering based sensor for glucose was developed with oligonucleotide functionalized gold nanoparticles (Oligo-AuNPs). Oligonucleotide 5'-SH-(A)(12)-AGACAAGAGAGG-3' (Oligo 1) modified AuNPs and oligonucleotide 5'-CAACAGAGAACG-(A)(12)-HS-3' (Oligo 2) modified AuNPs could hybridize with oligonulceotide 5'-CGTTCTCTGTTGCCTCTCTTGTCT-3' (Oligo 3), which resulted in the aggregation of Oligo-AuNPs probes, and triggered the increase of their average diameter. However, Oligo 3 could be cleaved into DNA fragments by the mixture of glucose, glucose oxidase (GOD) and Fe2+, and the DNA fragments could not hybridize with Oligo-AuNPs probes. Under the conditions of 3.7 nM Oligo 1-AuNPs, 3.7 nM Oligo 2-AuNPs, 8.0 mu g/mL GOD, 100 nM Oligo 3 and 900 nM Fe2+, the average diameter of Oligo-AuNPs probes decreased linearly with the increasing concentration of glucose over the range from 50 pmol/L to 5.0 nmol/L, with a detection limit of 38 pmol/L (3 sigma/slope). Moreover, five sugars had no effect on the average diameter of mixture of Oligo-AuNPs probes, GOD and Fe2+, which demonstrated the good selectivity of the assay. (C) 2012 Elsevier B.V. All rights reserved.