Effect of chronic verapamil treatment on ventricular function and growth in chick embryos.
Effect of chronic verapamil treatment on ventricular function and growth in chick embryos.
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长期维拉帕米治疗对鸡胚胎心室功能和生长的影响。
DOI：
10.1152/ajpheart.1991.261.1.h166
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Hansen,J
中科院分区：
文献类型：
--
作者：
Clark,EB;Hu,N;Turner,DR;Litter,JE;Hansen,J
心肌质量对工作负荷的调节是心脏的基本特征。本文研究了钙通道阻滞剂维拉帕米对鸡胚心室生长和功能的影响。我们用微渗透泵将维拉帕米输送到胚外血管床，并将其与盐水处理的对照和未处理的胚胎进行比较。在第24、27和29阶段，我们通过伺服零位系统测量心室压力和dP/dt，通过脉冲多普勒测量背主动脉每搏输出量和dV/dt，以及心室和胚胎湿重。通过数字测面技术测量平均肌细胞轮廓面积，并通过DNA和蛋白质分析测量细胞生长反应。与生理盐水对照组和正常胚胎相比，维拉帕米治疗降低了实验胚胎的心室压（P <0.05）;在第27阶段，分别为1.59 +/- 0.21 vs. 2.17 +/- 0.05和2.35 +/- 0.08（SE）mmHg。与对照组和正常胚胎相比，实验组（P <0.05）的平均背主动脉血流量降低;在第27期，分别为0.98 +/- 0.07 vs. 1.54 +/- 0.10和1.56 +/- 0.07 mm 3/s。除第29期外，所有实验、正常和对照胚胎的每搏输出量均保持不变。与对照和正常胚胎相比，实验胚胎的心室重量降低（P <0.05）;在第27阶段，分别为1.09 +/- 0.07 vs. 1.51 +/- 0.08和1.54 +/- 0.11 mg。所有组的胚胎重量、肌细胞大小和细胞质体积分数相似。脑室形态正常。与对照组和正常胚胎相比，实验胚胎的DNA含量较低（P <0.05）。（250字处删节）
Adjustment of myocardial mass to work load is a fundamental characteristic of the heart. We studied the effect of verapamil, a calcium channel blocker, on growth and function of chick embryonic ventricle. We treated stage 18 chick embryos with verapamil delivered to the extraembryonic vascular bed by a miniosmotic pump and compared them with saline-treated control and untreated embryos. At stages 24, 27, and 29, we measured ventricular pressure and dP/dt by a servo-null system, dorsal aortic stroke volume and dV/dt by pulsed-Doppler, and ventricular and embryo wet weights. Mean myocyte profile area was measured by digital planimetry technique, and cell growth response by DNA and protein assay. Verapamil treatment decreased ventricular pressure in experimental (P less than 0.05) compared with saline control and normal embryos; at stage 27, 1.59 +/- 0.21 vs. 2.17 +/- 0.05 and 2.35 +/- 0.08 (SE) mmHg, respectively. Mean dorsal aortic blood flow decreased in experimental (P less than 0.05) vs. control and normal embryos; at stage 27, 0.98 +/- 0.07 vs. 1.54 +/- 0.10 and 1.56 +/- 0.07 mm3/s, respectively. Stroke volume remained the same in all experimental, normal, and control embryos except at stage 29. Ventricular weight decreased in experimental (P less than 0.05) vs. control and normal embryos; at stage 27, 1.09 +/- 0.07 vs. 1.51 +/- 0.08 and 1.54 +/- 0.11 mg, respectively. Embryo weights, myocyte size, and cytoplasmic fractional volume were similar in all groups. Morphology of ventricles was normal. DNA was lower in experimental (P less than 0.05) compared with control and normal embryos.(ABSTRACT TRUNCATED AT 250 WORDS)