In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure.
In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure.
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DOI：
10.1364/ol.36.001029
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发表时间：
2011-04-01
期刊：
影响因子：
3.6
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Wang Y;Hu S;Maslov K;Zhang Y;Xia Y;Wang LV
开发了一种集成光声显微镜和荧光共聚焦显微镜的双模态显微镜，以高空间分辨率在单个血管中无创成像血红蛋白氧饱和度（sO2）和氧分压（pO 2）。虽然光声显微镜测量sO2成像血红蛋白在两个波长的光吸收，荧光共聚焦显微镜定量pO 2使用磷光淬灭。在高氧（100%氧）和常氧（21%氧）条件下，多个血管分支的sO2和pO 2值的变化与氧-血红蛋白解离曲线相关性良好。此外，血红蛋白的总浓度通过光声显微镜在等吸光波长下成像。
A dual-modality microscope integrating photoacoustic microscopy and fluorescence confocal microscopy was developed to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While photoacoustic microscopy measures sO2 by imaging hemoglobin optical absorption at two wavelengths, fluorescence confocal microscopy quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by photoacoustic microscopy at an isosbestic wavelength.