Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin
Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin
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DOI：
10.1016/s0304-3835(99)00188-3
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发表时间：
1999-09-20
期刊：
影响因子：
9.7
通讯作者：
Lee, BH
中科院分区：
文献类型：
--
作者：
Lee, SJ;Sung, JH;Lee, BH
研究了一种新型人参皂苷代谢产物20-O-β-D-吡喃葡萄糖基-20（S）-原人参二醇（IH-901）对4种人癌细胞系和1种顺铂（CDDP）耐药细胞亚系的体外抗肿瘤活性。MTT法测定化合物的生长抑制活性。IH-901对人髓性白血病（HL-60）、肺腺癌（PC-14）、胃腺癌（MKN-45）和肝癌HepG 2细胞系抑制50%细胞增殖所需的平均浓度（IC 50）分别为24.3、25.9、56.6和24.9 μ M。这些值高于CDDP。在耐CDDP的PC/DDP细胞系中，IH-901和CDDP的IC 50值分别为20.3和60.8 μ M。这些结果表明，IH-901在该细胞系中对CDDP没有交叉耐药，并且可能是治疗CDDP耐药肺癌的候选物。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
The in vitro antitumor activity of a novel ginseng saponin metabolite, 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol (IH-901), was examined against four human cancer cell lines and one subline resistant to cisplatin (CDDP). The growth inhibitory activity of the compound was estimated by MTT tetrazolium assay. The mean concentrations of IH-901 needed to inhibit the proliferation of the cells by 50% (IC50) were 24.3, 25.9, 56.6 and 24.9 mu M against human myeloid leukemia (HL-60), pulmonary adenocarcinoma (PC-14), gastric adenocarcinoma (MKN-45) and hepatoma HepG2) cell lines, respectively. These values are higher than that of CDDP. In the CDDP-resistant PC/DDP cell line, the IC50 values of IH-901 and CDDP were 20.3 and 60.8 mu M, respectively. These results suggest that IH-901 is not cross-resistant to CDDP in this cell line and could be a candidate for the treatment of CDDP resistant pulmonary cancer. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.