A high diffusion coefficient for coenzyme Q10 might be related to a folded structure
A high diffusion coefficient for coenzyme Q10 might be related to a folded structure
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DOI：
10.1016/s0014-5793(98)00313-5
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发表时间：
1998-04-10
期刊：
影响因子：
3.5
通讯作者：
Lenaz, G
中科院分区：
文献类型：
--
作者：
Di Bernardo, S;Fato, R;Lenaz, G
我们利用芘衍生物的荧光碰撞猝灭技术测量了不同辅酶Q同系物和类似物在模型脂质囊泡中的横向扩散，发现扩散系数在10(-6) cm(2)/s范围内，根据自由体积理论计算了这些高度疏水性组分的理论扩散系数。自由体积理论中的重要参数是扩散剂和溶剂之间的相对尺寸：辅酶的分子动力学计算机模拟产生了它们最可能的几何形状和体积，并揭示了短同系物和长同系物的惊人相似的尺寸，这是由于后者中类异戊二烯链的折叠结构，辅酶 Q(10) 的长度为 21 埃。利用这些信息，我们能够计算出 10(-6) cm(2)/s 范围内的扩散系数，与实验发现的结果非常一致。 (C) 1998 年欧洲生化学会联合会。
We measured the lateral diffusion of different coenzyme Q homologues and analogues in model lipid vesicles using the fluorescence collisional quenching technique with pyrene derivatives and found diffusion coefficients in the range of 10(-6) cm(2)/s, Theoretical diffusion coefficients for these highly hydrophobic components were calculated according to the free volume theory. tin important parameter in the free volume theory is the relative dimension between diffusant and solvent: a molecular dynamics computer simulation of the coenzymes yielded their most probable geometries and volumes and revealed surprisingly similar sizes of the short and long homologues, due to a folded structure of the isoprenoid chain in the latter, with a length for coenzyme Q(10) of 21 Angstrom. Using this information we were able to calculate diffusion coefficients in the range of 10(-6) cm(2)/s, in good agreement with those found experimentally. (C) 1998 Federation of European Biochemical Societies.