COATED VESICLES FROM PIG BRAIN - PURIFICATION AND BIOCHEMICAL CHARACTERIZATION
COATED VESICLES FROM PIG BRAIN - PURIFICATION AND BIOCHEMICAL CHARACTERIZATION
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DOI：
10.1016/s0022-2836(75)80024-6
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发表时间：
1975-01-01
影响因子：
5.6
通讯作者：
PEARSE, BMF
中科院分区：
文献类型：
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作者：
PEARSE, BMF
各种组织的电子显微镜已经揭示了细胞内囊泡的存在，在其细胞质表面上有“外套”。这种小泡与微胞饮作用有关。本文报道了一种简便、快速的纯化猪脑包被囊泡的方法。所获得的结构具有约600 μ m的外径和显著的网络状外观。本文报道的关于这些囊泡的最引人注目的观察结果是，它们基本上只含有一种蛋白质，在十二烷基硫酸钠凝胶上的表观分子量约为180，000。用胰蛋白酶或链霉蛋白酶消化外壳表明，大多数这种蛋白质位于这些结构的外表面（即面向细胞质）。由此释放的脂质囊泡似乎具有约350 μ m的外径。每个囊泡的蛋白质分子的数量的估计是一致的外壳具有二十面体排列的亚基。
Electron microscopy of various tissues has revealed the existence of intracellular vesicles with “coats” on their cytoplasmic surfaces. Such vesicles have been seen associated with micropinocytosis. A simple and rapid procedure for the purification of coated vesicles from pig brain has been developed. The structures obtained have an external diameter of about 600 Å and a remarkable network-like appearance. The most striking observation about these vesicles, reported here, is that they contain essentially just one protein species, having an apparent molecular weight on sodium dodecyl sulphate gels of about 180,000. Digestion of the coats with trypsin or Pronase shows that most of this protein is located on the external surface (i.e. facing the cytoplasm) of these structures. The lipid vesicles thus liberated appear to have an external diameter of about 350 Å. An estimate of the number of protein molecules per vesicle is consistent with the coat having an icosahedral arrangement of subunits.