Culture Factors Affecting the Success Rate of in Vitro Fertilization and Embryo Transfer a
Culture Factors Affecting the Success Rate of in Vitro Fertilization and Embryo Transfer a
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影响体外受精和胚胎移植成功率的培养因素
DOI：
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发表时间：
1985
影响因子：
5.2
通讯作者：
C. Kirby
中科院分区：
文献类型：
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作者：
Patrick Quinn;G. Warnes;J. Kerin;C. Kirby
单细胞小鼠受精卵在体外发育至囊胚阶段已被用作人体外受精和胚胎移植（IVF/ET）所用培养基和处理程序的质量控制。将单细胞小鼠受精卵置于含有牛血清白蛋白的培养基中培养。取同批含女性患者同源血清的培养液进行人卵母细胞的受精和培养。以下程序与IVF/ET后小鼠胚胎发育和人类妊娠的高比率相关：培养基的充分充气和平衡，用于处理胚胎的移液管和导管的双重冲洗，在不存在含有5%CO2的气氛的情况下使用HEPES缓冲培养基来操作胚胎，控制胚胎附近的过高温度，ET采用含50%患者血清的培养基。这些程序的实施使怀孕率更加稳定。然而，小鼠胚胎发育试验确定的人卵母细胞的受精和卵裂与培养液的质量之间没有明显的关联。在一项持续的试验中，我们比较了两种培养基（改良的台氏培养基和根据人输卵管液[HTF]组成配制的培养基）和两种培养技术（在培养皿中的油下培养基中培养和在松盖试管中培养）。与台氏培养基相比，HTF培养基中发育的小鼠受精卵显著更多。在人类IVF/ET的随机2 × 2析因试验中，当在油下的HTF培养基中进行受精和培养时，妊娠率最高，但数量尚不足以显示治疗之间的任何统计学差异。
The development of one-cell mouse zygotes to the blastocyst stage in vitro has been used as a quality control for the media and handling procedures employed for human in vitro fertilization and embryo transfer (IVF/ET). One-cell mouse zygotes were placed in culture in medium containing bovine serum albumin. Aliquots of the same batch of medium containing female patients' homologous serum were used for the fertilization and culture of human oocytes. The following procedures were associated with high rates of mouse embryo development and human pregnancies following IVF/ET: adequate gassing and equilibration of the medium, double-rinsing of pipets and catheters used to handle embryos, use of a HEPES-buffered medium for manipulating embryos in the absence of an atmosphere containing 5% CO2, control of excessive temperature in the vicinity of the embryos, and ET using medium containing 50% patient's serum. The institution of these procedures gave more consistent pregnancy rates. However, there was no obvious association between fertilization and cleavage of human oocytes and the quality of the medium ascertained by the mouse embryo development test. In a continuing trial, we are comparing two culture media (modified Tyrode's and a medium formulated on the composition of human fallopian tube fluid [HTF]) and two culture techniques (culture in medium under oil in petri dishes and in loosely capped tubes). Significantly more mouse zygotes developed in HTF medium compared to Tyrode's medium. In a randomized 2 X 2 factorial trial with human IVF/ET, the highest pregnancy rate occurred when fertilization and culture were carried out in HTF medium under oil, but numbers are not yet sufficient to show any statistical difference between treatments.