DNA methylation of NR3c1 in infancy: Associations between maternal caregiving and infant sex
DNA methylation of NR3c1 in infancy: Associations between maternal caregiving and infant sex
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DOI：
10.1002/imhj.21789
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发表时间：
2019-07-01
影响因子：
2.4
通讯作者：
Lester, Barry M.
中科院分区：
文献类型：
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作者：
Conradt, Elisabeth;Ostlund, Brendan;Lester, Barry M.
照顾者在支撑婴儿应激反应和调节中起着关键作用，但这种支撑发生的机制尚不清楚。动物模型强烈表明，表观遗传过程，如DNA甲基化，是敏感的行为，反过来，后代的压力反应。我们研究了婴儿的哺乳行为对婴儿DNA甲基化和婴儿应激反应的直接影响。婴儿和母亲（N = 128）在婴儿5个月大时自由玩耍期间进行了评估。母亲的反应和适当的触摸编码。和婴儿颊上皮细胞取样，以评估糖皮质激素受体基因NR3c1外显子1F的DNA甲基化。婴儿皮质醇的反应性进行了评估，以应对静止的脸范例。较高水平的母亲反应和适当的触摸与NR3c1外显子1F特定区域的DNA甲基化较少有关，但仅限于女性。母亲的反应性和适当的触摸或NR3c1外显子1F的DNA甲基化对预应力皮质醇或皮质醇反应性没有关联。我们的研究结果进行了讨论，涉及产妇护理的一个重要因素，在编程婴儿的压力反应的编程模型。
Caregivers play a critical role in scaffolding infant stress reactivity and regulation, but the mechanisms by which this scaffolding occurs is unclear. Animal models strongly suggest that epigenetic processes, such as DNA methylation, are sensitive to caregiving behaviors and, in turn, offspring stress reactivity. We examined the direct effects of caregiving behaviors on DNA methylation in infants and infant stress reactivity. Infants and mothers (N = 128) were assessed during a free play when infants were 5 months old. Maternal responsiveness and appropriate touch were coded. and infant buccal epithelial cells were sampled to assess for DNA methylation of the glucocorticoid receptor gene, NR3c1 exon 1F. Infant cortisol reactivity was assessed in response to the still-face paradigm. Greater levels of maternal responsiveness and appropriate touch were related to less DNA methylation of specific regions in NR3c1 exon 1F, but only for females. There was no association with maternal responsiveness and appropriate touch or DNA methylation of NR3c1 exon 1F on prestress cortisol or cortisol reactivity. Our results are discussed in relation to programming models that implicate maternal care as an important factor in programing infant stress reactivity.