Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta.
Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta.
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DOI：
10.1016/j.bbrc.2005.02.186
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发表时间：
2005-05
影响因子：
3.1
通讯作者：
Mari Tomiie;Shigeyuki Isaka;E. Miyoshi;N. Taniguchi;T. Kimura;K. Ogita;T. Tsutsui;K. Shimoya;T. Nakagawa;A. Kondo;M. Koyama;Y. Murata
中科院分区：
文献类型：
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作者：
Mari Tomiie;Shigeyuki Isaka;E. Miyoshi;N. Taniguchi;T. Kimura;K. Ogita;T. Tsutsui;K. Shimoya;T. Nakagawa;A. Kondo;M. Koyama;Y. Murata
在妊娠早期，胎盘滋养层细胞迅速生长并侵入母体子宫组织。N-乙酰氨基葡萄糖转移酶V(GnT-V)及其产物β1-6-GlcNAc分支糖链与肿瘤的侵袭和转移密切相关。由于胎盘形成过程类似于癌细胞的侵袭，我们检测了β1-6-GlcNAc分支糖和GnT-V在人胎盘中的表达。用白血球凝集素凝集素印迹法检测，妊娠早期胎盘中含有较多的β1-6-GlcNAc分枝多糖。免疫组织化学研究表明，β1-6-GlcNAc分支糖链和GnT-V蛋白定位于滋养层。早孕胎盘中GnT-V的蛋白表达和酶活性均高于足月胎盘。这些结果提示，GnT-V可能参与了妊娠早期的胎盘形成，可能调节滋养层细胞的侵袭过程。
In early pregnancy, placental trophoblast cells rapidly grow and invade into maternal uterine tissue. N-Acetylglucosaminyltransferase V (GnT-V) and its product, β1-6-GlcNAc branching glycan, are known to correlate with tumor invasion and metastasis. Since the placentation process resembles invasion of cancer cells, we examined the expression of β1-6-GlcNAc branching glycan and GnT-V in human placenta. Placentas derived from the first trimester contained a larger amount of β1-6-GlcNAc branching glycan, detected by leukoagglutinating phytohemagglutinin lectin blotting, than those at term. Immunohistochemical study revealed that β1-6-GlcNAc branching glycans and GnT-V protein were localized in the trophoblast layer. Both protein expression and the enzyme activity of GnT-V in first trimester placentas were higher than those at term. These results suggest that GnT-V would contribute to placentation in the early phase of pregnancy, possibly regulating the process of invasion of trophoblast cells.