A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus
A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus
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DOI：
10.1371/journal.pone.0058931
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发表时间：
2013-03-14
期刊：
影响因子：
3.7
通讯作者：
Idnurm, Alexander
中科院分区：
文献类型：
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作者：
Chaudhary, Suman;Polaino, Silvia;Idnurm, Alexander
Blakesleeanus是Mucoromycotina亚门的成员。用134个标记构建了布莱克松疫霉两个野生型菌株杂交后代的遗传图谱。这些标记主要是PCR-RFLP。标记位于基因组序列的46个支架上，覆盖了超过97%的基因组。在后代中的等位基因的分析揭示了9个或12个连锁群，这取决于对数的赔率（LOD）的分数，跨越1583.4 cM在LOD 5。连锁群覆盖在先前的映射数据从突变体之间的交叉，辅助的新鉴定的突变在初级代谢突变株。分子标记图谱、表型图谱和基因组序列总体上是一致的，但有一些例外。新的遗传图谱提供了全基因组重组估计，平均每厘米33.2 kb。这种频率是毛霉亚门物种接合孢子发育过程中减数分裂的一个证据。在减数分裂的同时，非重组染色体的传递在藻属的交配过程中也很明显。新的图谱提供了基因组序列的支架排序和一个平台，在此平台上，使用定位克隆来鉴定受感兴趣的性状影响的突变体中的基因，如胡萝卜素生物合成，向光性或向重力性。
Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCR-RFLPs. Markers were located on 46 scaffolds of the genome sequence, covering more than 97% of the genome. Analysis of the alleles in the progeny revealed nine or 12 linkage groups, depending on the log of the odds (LOD) score, across 1583.4 cM at LOD 5. The linkage groups were overlaid on previous mapping data from crosses between mutants, aided by new identification of the mutations in primary metabolism mutant strains. The molecular marker map, the phenotype map and the genome sequence are overall congruent, with some exceptions. The new genetic map provides a genome-wide estimate for recombination, with the average of 33.2 kb per cM. This frequency is one piece of evidence for meiosis during zygospore development in Mucoromycotina species. At the same time as meiosis, transmission of non-recombinant chromosomes is also evident in the mating process in Phycomyces. The new map provides scaffold ordering for the genome sequence and a platform upon which to identify the genes in mutants that are affected in traits of interest, such as carotene biosynthesis, phototropism or gravitropism, using positional cloning.